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008			180820s2018 xxua o |||| 0|eng d
020			_a9781493987191
024	7		_a10.1007/978-1-4939-8719-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH601 _b2018 EB
245	0	0	_aClathrin-Mediated Endocytosis : _bMethods and Protocols _cedited by Laura E. Swan
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 253 páginas) _b51 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1847
505	0		_aPurification of Clathrin-coated Vesicles from Adult Rat Brain -- Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation -- Probing Endocytosis during the Cell Cycle with Minimal Experimental Perturbation -- Assaying the Contribution of Membrane Tension to Clathrin-mediated Endocytosis -- Identifying Small Molecule Inhibitors of the Clathrin Terminal Domain -- Acute Manipulations of Clathrin-mediated Endocytosis at Presynaptic Nerve Terminals -- Imaging "Hot-wired" Clathrin-mediated Endocytosis -- Real-time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals -- Assaying Mutants of Clathrin Mediated Endocytosis in the Fly Eye -- Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-molecule Analysis -- Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in Isolated Plasma Membranes -- SMrT Assay for Real-time Visualization and Analysis of Clathrin Assembly Reactions -- Real-time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System -- Stimulated Emission Depletion (STED) Imaging of Clathrin-mediated Endocytosis in Living Cells -- Measuring Clathrin-coated Vesicle Formation with Single-molecule Resolution -- Cryo-electron Tomography of the Mammalian Synapse -- Quantitative Analysis of Clathrin-mediated Endocytosis in Yeast by Live Cell Fluorescence Microscopy -- Using FM Dyes to Monitor Clathrin-mediated Endocytosis in Primary Neuronal Culture.
520			_aThis book presents a methodological introduction on clathrin-mediated endocytosis. Chapters guide readers through a number of model systems, purifications, reconstitutions, analysis techniques and systemic alterations of membrane function, which has been pivotal in understanding the role of clathrin and is panoply of interactors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clathrin-Mediated Endoytsis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2018
650		7	_2embne _9142266 _aMembranas celulares
650		7	_2embne _9138639 _aBioquímica
776	0	8	_iPrinted edition: _z9781493987177
776	0	8	_iPrinted edition: _z9781493987184
776	0	8	_iPrinted edition: _z9781493993741
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8719-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI