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020 _a9781493908820
024 7 _a10.1007/978-1-4939-0882-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRB155
_b2014 EB
245 0 0 _aInnate DNA and RNA Recognition :
_bMethods and Protocols
_cedited by Hans-Joachim Anders, Adriana Migliorini
250 _a1st edition 2014
264 1 _aNew York, NY
_bSpringer International Publishing
_c2014
300 _a1 recurso en línea (XI, 195 páginas)
_b37 ilustraciones, 16 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1169
505 0 _aDetection of RNA Modifications by HPLC Analysis and Competitive ELISA -- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand -- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis -- Isolation of RIG-I-associated RNAs from Virus Infected Cells -- Structure Modeling of Toll-like Receptors -- Nucleic Acid Recognition in Dendritic Cell -- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems -- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro -- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes -- Mapping of Optimal CD8 T Cell Epitopes -- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection -- MicroRNA Methodology: Advances in miRNA-technologies -- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) -- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus -- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice -- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice -- Bifunctional siRNAs for Tumor Therapy.
520 _aInnate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists, and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.
988 _aSpringer_Protocols_2014
650 7 _2embne
_9144879
_aGenética médica
650 7 _2embne
_9140562
_aGenética humana
776 0 8 _iPrinted edition:
_z9781493908837
776 0 8 _iPrinted edition:
_z9781493908813
776 0 8 _iPrinted edition:
_z9781493945832
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0882-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b05/2023
_dz
_eb
_zSI