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| 008 | 100301s1998 xxu| o |||| 0|eng d | ||
| 020 | _a9781592595655 | ||
| 024 | 7 |
_a10.1385/0896034488 _2doi |
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_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC |
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_aQP801 .P638 _b1998 EB |
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| 245 | 0 | 0 |
_aPolyamine Protocols _cedited by David M. L. Morgan |
| 250 | _a1st edition 1998 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c1998 |
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| 300 | _a1 recurso en línea (IX, 186 páginas) | ||
| 336 |
_aTexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
||
| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v79 |
|
| 505 | 0 | _aPolyamines: An Introduction -- Assay Methods for Enzymes of Polyamine Biosynthesis -- Determination of Ornithine Decarboxylase Activity Using [3H]Omithine -- Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine -- Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity -- Assay of Spermidine and Spermine Synthases -- Measurement of Spermidine/Spermine N 1-Acetyltransferase Activity -- Assay of Spermidine N 8-Acetyltransferase -- Assay Methods for Enzymes of Polyamine Catabolism -- Assay of N 8-Acetylspermidine Deacetylase -- Hydrogen Peroxide Assay for Amine Oxidase Activity -- Radiochemical Assay of Diamine Oxidase -- Radiochemical Estimation of Polyamine Oxidase -- Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity -- Measurement of Polyamines -- Determination of Polyamines as Their Benzoylated Derivatives by HPLC -- A Dansyl Chloride-HPLC Method for the Determination of Polyamines -- The Determination of Polyamines and Amino Acids by a Fluorescamine-HPLC Method -- Thin-Layer Chromatographic Method for Assaying Polyamines -- Measurement of Polyamine Transport -- Measurement of Polyamine Transport: Adherent Cells -- Measurement of Polyamine Transport: Cells in Suspension -- Measurement of Polyamine Efflux from Cells in Culture -- Measurement of Polyamine Effects on Cell Growth -- Leucine Incorporation and Thymidine Incorporation -- Tetrazolium (MTT) Assay for Cellular Viability and Activity. | |
| 520 | _aDavid Morgan brings together in Polyamine Protocols a unique collection of hands-on enzyme assay techniques for the study of polyamines and their biological functions. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. All methods are presented by active researchers who have developed them to a high degree of perfection in their own laboratories, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results. Polyamine Protocols is the only compilation of methods currently available for use in studies involving polyamines. The collection offers both novice and experienced researchers across many fields-including cell and molecular biology, pharmacology, oncology, physiology, laboratory medicine, and clinical pathology-all the tools needed to elucidate the biological roles played by these ubiquitous substances. | ||
| 988 | _aSpringer_Protocols_1998 | ||
| 650 | 7 |
_2embne _9147296 _aPoliaminas _xMetabolismo |
|
| 776 | 0 | 8 |
_iPrinted edition: _z9781617370427 |
| 776 | 0 | 8 |
_iPrinted edition: _z9780896034488 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489942739 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034488 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid) |
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_b05/2023 _dz _eb _zSI |
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