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007			cr nn 008mamaa
008			111201s2012 xxu| o |||| 0|eng d
020			_a9781617794155
024	7		_a10.1007/978-1-61779-415-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.2 _b2012 EB
245	0	0	_aBone Research Protocols _cedited by Miep H. Helfrich, Stuart H. Ralston
250			_a2nd edition 2012
264		1	_aTotowa, N.J. _bHumana Press _c2012
300			_a1 recurso en línea (XIV, 643 páginas) _b152 ilustraciones, 39 ilustraciones a color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v816
505	0		_aPrimary Human Osteoblast Cultures -- Osteoblast Isolation from Murine Calvaria and Long Bones -- Rat Osteoblast Cultures -- Isolation of primary avian Osteocytes -- Isolation of Mouse Osteocytes using Cell Fractionation for Gene Expression Analysis -- Studying Osteocyte Function using the Cell Lines MLO-Y4 and MLO-A5 -- Isolation, Differentiation and Characterisation of Human Bone Marrow Stem Cells in vitro and in vivo -- Rodent Osteoclast Cultures -- Isolation and Culture of Primary Chicken Osteoclasts -- ISOLATION AND PURIFICATION OF RABBIT OSTEOCLASTS -- Generation of Human Osteoclasts from Peripheral Blood -- Osteoclast Formation in Mouse Co-Cultures -- Rankl- Mediated Osteoclast Formation from Murine RAW 264.7 Cells -- Transfection of Osteoclasts and Osteoclast Precursors -- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation -- Analysis of Transcriptional Regulation in Bone Cells -- Extraction of Nucleic Acids from Bone -- Analysis of Gene Expression in Bone by Quantitative RT/PCR -- Histomorphometry in Rodents -- Studying Gene Expression in Bone by in situ Hybridization -- Immunostaining of Skeletal Tissue -- Techniques for the Study of Apoptosis in Bone -- Transmission Electron Microscopy of Bone -- Scanning Electron Microscopy of Bone -- Fluorescence Imaging of Osteoclasts using Confocal Microscopy -- Live Imaging of Bone Cell and Organ Cultures -- Analysis of Bone Architecture in Rodents using Micro Computed Tomography -- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents -- Quantitative X-ray Imaging of Rodent Bone by Faxitron -- Bioluminescence Imaging of Bone Metastasis in Rodent -- Fourier Transform Infrared Imaging of Bone -- Raman Microscopy of Bone -- The Calvarial Injection Assay -- Ovariectomy/Orchidectomy in Rodents -- Mechanical Properties of Bone ex vivo -- Mechanical Stimulation of Bone Cells using Fluid Flow -- using CELL and organ culture models TO analyse responses OF bone cellS to mechanical STIMULATION .In vivo Mechanical Loading.
520			_aStudies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.
988			_aSpringer_Protocols_2012
650		7	_2embne _9666561 _aHuesos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617794148
776	0	8	_iPrinted edition: _z9781617794162
776	0	8	_iPrinted edition: _z9781493961009
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-415-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI