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008			131204s2014 xxu| o |||| 0|eng d
020			_a9781627035927
024	7		_a10.1007/978-1-62703-592-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981 _b2014 EB
245	0	0	_aPlant Chemical Genomics : _bMethods and Protocols _cedited by Glenn R. Hicks, Stéphanie Robert
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 269 páginas) _b169 ilustraciones, 13 ilustraciones a color
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1056
505	0		_aFully-automated Compound Screening in Arabidopsis thaliana Seedlings -- Time-profiling Fluorescent Reporters in the Arabidopsis Root -- Screening for Bioactive Small Molecules by in vivo Monitoring of Luciferase-based Reporter Gene Expression in Arabidopsis thaliana -- Application of Yeast-two Hybrid Assay to Chemical Genomic Screens: A High-throughput System to Identify Novel Molecules Modulating Plant Hormone Receptor Complexes -- High-throughput Screening of Small Molecule Libraries for Inducers of Plant Defense Responses -- Using a Reverse Genetics Approach to Investigate Small Molecule Activity -- Investigating the Phytohormone Ethylene Response Pathway by Chemical Genetics -- Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in membrano and in Planta -- Forward Chemical Screening of Small RNA Pathways -- Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening -- High-throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen -- Plant Chemical Genomics: Gravity Sensing and Response -- Screening Chemical Libraries for Compounds that Affect Protein Sorting to the Yeast Vacuole -- The use of Multi-drug Approach to Uncover New Players of the Endomembrane System Trafficking Machinery -- Cheminformatic Analysis of High-Throughput Compound Screens -- Endomembrane Dissection Using Chemically-Induced Bioactive Clusters -- Statistical Molecular Design: A Tool to Follow Up Hits From Small Molecule Screening -- Early Stage Hit Triage for Plant Chemical Genetic Screens and Target Site Identification -- Screening for Gene Function using the FOX (Full-length cDNA OvereXpressor gene) Hunting System -- Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry -- 1H NMR Based Metabolomics Methods for Chemical Genomics Experiments -- Determination of Auxin Transport Parameters on the Cellular Level -- Analyzing the in vivo Status of Exogenously Applied Auxins: A HPLC-based Method to Characterize the Intracellularly Localized Auxin Transporters.
520			_aPlant Chemical Genomics: Methods and Protocols , expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall. The presentation of these and other examples as well as synthetic chemistry, computation and target identification provides a comprehensive overview of the practical aspects of chemical biology that are possible in plant systems. Small molecules and natural products that produce a phenotype in plants have been known for decades. The agrichemical industry has utilized small molecules based on known natural molecules such as auxins, cytokinins, abscissic acid and other growth regulators. In recent years, the screening and characterization of novel small molecules has enhanced our understanding of protein function, metabolic, signaling and endomembrane pathways, and their interactions in basic plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
988			_aSpringer_Protocols_2014
650		7	_2embne _9139640 _aGenética vegetal
650		7	_2embne _9139638 _aFitoquímica
776	0	8	_iPrinted edition: _z9781627035934
776	0	8	_iPrinted edition: _z9781627035910
776	0	8	_iPrinted edition: _z9781493959518
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-592-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI