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999			_c390930 _d390930 _x1
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007			cr nn 008mamaa
008			100301s1997 xxu| o |||| 0|eng d
020			_a9781592596331
024	7		_a10.1385/0896034577 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP376 _b1997 EB
100	1		_aSeidman, Shlomo _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688504
245	1	0	_aTransgenic Xenopus : _bMicroinjection Methods and Developmental Neurobiology _cby Shlomo Seidman, Hermona Soreq
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (XVIII, 198 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v28
505	0		_aScientific Background -- Experimental Methodologies -- Experimental Applications -- Conclusions
520			_aThe need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system's ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal.
988			_aSpringer_Protocols_1997
650		7	_2embne _9139532 _aCerebro _xFisiología
650		7	_2embne _9160713 _aDesarrollo neurológico
650		7	_2embne _9138501 _aAnfibios _xGenética
700	1		_aSoreq, H. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688505
776	0	8	_iPrinted edition: _z9781617370458
776	0	8	_iPrinted edition: _z9781489940476
776	0	8	_iPrinted edition: _z9780896034570
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034577 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI