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008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592591374
024	7		_a10.1385/159259137X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC269.5 _b2001 EB
245	0	0	_aMetastasis Research Protocols _cedited by Susan A. Brooks, Udo Schumacher
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XXII, 310 páginas)
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v58
505	0		_aSelection/Segregation Cell Populations for in Vitro and in Vivo Assays of Metastatic Behavior -- Cell Separations by Flow Cytometry -- Immunomagnetic Cell Separation -- Genetic Modification of Cell Lines to Enhance Their Metastatic Capability -- In Vitro Assays of Metastatic Behavior -- Cell Aggregation Assays -- Cell Migration and the Boyden Chamber -- Quantification of Cell Motility -- In Vitro Invasion Assay Using Matrigel® -- Membrane Invasion Culture System -- Collagen Invasion Assay -- Chick Heart Invasion Assay -- Adhesion of Tumor Cells to Matrices and Endothelium -- Assessment of Angiogenic Factors The Chick Chorioallantoic Membrane Assay -- Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis -- Galectin-3 Binding and Metastasis -- Animal Models of Metastasis -- Basic Principles for the Study of Metastasis Using Animal Models -- The Chick Embryo Metastasis Model -- GeneTransfection for Metastasis Research Using Animal Models -- Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice -- Xenograft Models in Immunodeficient Animals -- Xenograft Models in Immunodeficient Animals -- Syngeneic Murine Metastasis Models -- Transgenic Animal Models -- Clinically Accurate Orthotopic Mouse Models of Cancer -- Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo -- Green Fluorescent Protein for Metastasis Research.
520			_aAlthough ninety percent of fatal cancer cases involve the spread of a primary tumor, the formation of metastases is still a poorly understood, complex process and a significant problem in the treatment of cancer patients. In Metastasis Research Protocols, leading international investigators describe in detail the key methods needed to investigate why and how metastasis occurs. Volume II of this two-volume set, Analysis of Cell Behavior In Vitro and In Vivo, presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and in whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis. The first volume of this set, Analysis of Cells and Tissues, focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. Comprehensive and authoritative, the two volumes of Metastasis Research Protocols constitute a gold-standard collection of readily reproducible methods for understanding the metastatic cascade-first at the cellular and molecular levels, then at the level of the whole organism-responsible for the spread of cancer and for developing novel strategies to combat its spread.
988			_aSpringer_Protocols_2001
650		7	_2embne _9413460 _aMetástasis
776	0	8	_iPrinted edition: _z9781617370991
776	0	8	_iPrinted edition: _z9780896036154
776	0	8	_iPrinted edition: _z9781489939074
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259137X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI