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008			101109s2011 xxu| o |||| 0|eng d
020			_a9781607619840
024	7		_a10.1007/978-1-60761-984-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585.2 _b2011 EB
245	0	0	_a3D Cell Culture : _bMethods and Protocols _cedited by John W. Haycock
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 343 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v695
505	0		_a3D Cell Culture: A Review of Current Approaches and Techniques -- Scaffolds for Tissue Engineering and 3D Cell Culture -- Tracking Nanoparticles in Three Dimensional Tissue Engineered Models Using Confocal Laser Scanning Microscopy -- Using Immuno-Scanning Electron Microscopy for the Observation of Cell Adhesion Interaction at the Nano- and Microscale in S-phase Cells -- 3D Sample Preparation for Orthopedic Tissue Engineering Bioreactors -- Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs -- Genetic Modification of Chondrocytes Using Viral Vectors -- Stem Cell and Neuron Co-Cultures for the Study of Nerve Regeneration -- Production of Tissue Engineered Skin and Oral Mucosa for Clinical and Experimental Use -- Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair -- Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization -- Micro-Structured Materials and Mechanical Cues in 3D Collagen Gels -- Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract -- Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial - Neuroectodermal - Mesenchymal Interaction System -- Human Endothelial and Osteoblast Co-Cultures on 3D Biomaterials -- Assessment of Nanomaterials Cytotoxicity and Internalization -- Practical Aspects of OCT Imaging in Tissue Engineering -- Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture -- 3D Structuring of Biocompatible and Biodegradable Polymers via Stereolithography -- Alvetex®: Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture.
520			_aDeveloped for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.
988			_aSpringer_Protocols_2011
650		7	_2embne _9139475 _aCélulas
776	0	8	_iPrinted edition: _z9781607619833
776	0	8	_iPrinted edition: _z9781607619857
776	0	8	_iPrinted edition: _z9781493957279
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-984-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI