000			04803nam a2200409 i 4500
999			_c390915 _d390915
001			390915
003			ES-MaUEC
005			20230907123535.0
006			a||||o|||| 00| 0
007			cr nn 008mamaa
008			100715s2001 xxu| o |||| 0|eng d
020			_a9781592590599
024	7		_a10.1385/1592590594 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP609 .P56 _b2001 EB
245	0	0	_aProtein Kinase Protocols _cedited by Alastair D. Reith
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XIII, 365 páginas)
336			_aTexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v124
505	0		_aProtein Kinase-Mediated Signaling Networks -- Cloning Protein Tyrosine Kinases by Screening cDNA Libraries with Antiphosphotyrosine Antibodies -- Immunoprecipitation and Western Blotting of Phosphotyrosine-Containing Proteins -- Two-Dimensional Phosphoamino Acid Analysis -- Two-Dimensional Phosphopeptide Mapping of Receptor Tyrosine Kinases -- Identification of the Sites of Phosphorylation in Proteins Using High Performance Liquid Chromatography and Mass Spectrometry -- Phosphorylation of Smad Signaling Proteins by Receptor Serine/Threonine Kinases -- Assays for Mitogen-Activated Protein Kinase (MARK) Subtypes and MARK Activating Protein Kinase-2 (MAPKAP K-2) Using a Common Cell Lysate -- JAK-Mediated Phosphorylation and Activation of STAT Signaling Proteins -- Assays for Glycogen Synthase Kinase-3(GSK-3) -- Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors -- Protein Histidine Kinase -- Cloning and Expression of Recombinant HEXA-HIS Tagged ZAP-70 Using the Baculovirus System -- Analysis of Protein Kinase Subcellular Localization by Visualization of GFP Fusion Proteins -- Detection of Phosphorylation-Dependent Interactions by Far-Western Gel Overlay -- Purification of Tyrosine-Phosphorylated Proteins by Immunoaffinity Chromatography and Direct Cloning of Their cDNAs from Bacterial Expression Libraries -- Identification of Receptor Tyrosine Kinase (RTK) Substrates by the Cloning of ReceptorTargets (CORT) Strategy -- Identification of Receptor Tyrosine Kinase Associating Proteins Using the Yeast Two-Hybrid System -- cDNA Expression Cloning and Characterization of Phosphorylation Dependent Protein Interactors Using the Yeast Tribrid System -- Analysis of SH2 Domain - Phosphopeptide Interactions by Isothermal Titration Calorimetry and Surface Plasmon Resonance -- Cloning and Characterization of RTK Ligands Using Receptor-Alkaline Phosphatase Fusion Proteins -- Isolation and Characterization of "Orphan-RTK" Ligands Using an Integrated Biosensor Approach.
520			_aAs key components of many cell signaling pathways, protein kinases are implicated in a broad variety of diseases, including cancers and neurodegenerative conditions, and offer considerable potential as tractable targets for therapeutic intervention. In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and provide more experienced investigators many novel time-saving ploys. Emphasis is given to the critical technical steps that are often omitted from methods published in the primary literature. There are also tips on potential pitfalls and copious notes on how to adjust the protocols to work in related systems. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.
988			_aSpringer_Protocols_2001
650		7	_2embne _9510854 _aProteína cinasas
650		7	_2embne _9141385 _aGenética molecular
776	0	8	_iPrinted edition: _z9781617371417
776	0	8	_iPrinted edition: _z9780896037007
776	0	8	_iPrinted edition: _z9781489938701
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590594 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI