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008			100715s2010 xxu| o |||| 0|eng d
020			_a9781607613763
024	7		_a10.1007/978-1-60761-376-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH583.2 _b2010 EB
245	0	0	_aCytoskeleton Methods and Protocols _cedited by Ray H. Gavin
250			_a2nd edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XII, 391 páginas) _b77 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v586
505	0		_aLive-Cell Imaging of the Cytoskeleton -- High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis -- Imaging the Cytoskeleton in Live Xenopus laevis Embryos -- Live-Cell Imaging of the Cytoskeleton and Mitochondrial-Cytoskeletal Interactions in Budding Yeast -- Dynamics of Actin Filaments and Microtubules -- Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization -- Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts -- Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components -- Quantitative Fluorescence Microscopy Techniques -- A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule -- Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots -- Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells -- Electron Microscopy Protocols for Imaging the Cytoskeleton -- Imaging Cytoskeleton Components by Electron Microscopy -- Purification and Localization of Intraflagellar Transport Particles and Polypeptides -- High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies -- Strategies for Imaging Microtubules in Plant Cells -- Analysis of Cell and Organelle Motility -- A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity -- Melanosome Motility in Fish Retinal Pigment Epithelial Cells -- Analysis of Properties of Cilia Using Tetrahymena thermophila -- Application of Cell Traction Force Microscopy for Cell Biology Research -- 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic and Proteomics Approaches for Cytoskeleton Protein Function -- The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions -- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila -- Proteomic Tools for the Analysis of Cytoskeleton Proteins.
520			_aIn the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology™ series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls. Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.
988			_aSpringer_Protocols_2010
650		7	_2embne _9139477 _aCitología _vManuales de laboratorio
650		7	_2embne _9138639 _aBioquímica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607613770
776	0	8	_iPrinted edition: _z9781607613756
776	0	8	_iPrinted edition: _z9781617796494
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-376-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eb _zSI