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007			cr nn 008mamaa
008			230503s2020 sz | s |||| 0|eng d
020			_a9783031023859
024	7		_a10.1007/978-3-031-02385-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH212.A78 _b2020 EB
100	1		_aTouhami, Ahmed _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9688325
245	1	0	_aAtomic Force Microscopy : _bA New Look at Microbes _cby Ahmed Touhami
250			_a1st edition 2020
264		1	_aCham _bSpringer International Publishing _c2020
300			_a1 recurso en línea (XIV, 97 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSynthesis Lectures on Materials and Optics _x2691-1949
505	0		_aPreface -- Acknowledgments -- Measurement Methods in Atomic Force Microscopy -- Cell Surface Structures at the Nanoscale -- AFM Force Spectroscopy of Living Bacteria -- Bacteria Mechanics at the Nanoscale -- Author's Biography.
520			_aOver the last two decades, Atomic Force Microscopy (AFM) has undoubtedly had a considerable impact in unraveling the structures and dynamics of microbial surfaces with nanometer resolution, and under physiological conditions. Moreover, the continuous innovations in AFM-based modalities as well as the combination of AFM with modern optical techniques, such as confocal fluorescence microscopy or Raman spectroscopy, increased the diversity and volume of data that can be acquired in an experiment. It is evident that these combinations provide new ways to investigate a broad spectrum of microbiological processes at the level of single cells. In this book, I have endeavored to highlight the wealth of AFM-based modalities that have been implemented over the recent years leading to the multiparametric and multifunctional characterization of, specifically, bacterial surfaces. Examples include the real-time imaging of the nanoscale organization of cell walls, the quantification of subcellular chemical heterogeneities, the mapping and functional analysis of individual cell wall constituents, and the probing of the nanomechanical properties of living bacteria. It is expected that in the near future more AFM-based modalities and complementary techniques will be combined into single experiments to address pertinent problems and challenges in microbial research. Such improvements may make it possible to address the dynamic nature of many more microbial cell surfaces and their constituents, including the restructuring of cellular membranes, pores and transporters, signaling of cell membrane receptors, and formation of cell-adhesion complexes. Ultimately, manifold discoveries and engineering possibilities will materialize as multiparametric tools allow systems of increasing complexity to be probed and manipulated.
988			_aSynthesis Collection of Technology_2020
650		7	_2embne _9138719 _aMicroscopía
650		7	_2embne _9142266 _aMembranas celulares
776	0	8	_iPrinted edition: _z9783031002496
776	0	8	_iPrinted edition: _z9783031012570
776	0	8	_iPrinted edition: _z9783031035135
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-031-02385-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b04/2023 _dz _eIG _zSI