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007			cr nn 008mamaa
008			220601s2011 sz | s |||| 0|eng d
020			_a9783031025709
024	7		_a10.1007/978-3-031-02570-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aR857.B54 _b2011 EB
100	1		_aMeer, Jan Roelof van der _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9687757
245	1	0	_aBacterial Sensors _bSynthetic Design and Application Principles _cby Jan Roelof Meer.
250			_a1st edition 2011
264		1	_aCham _bSpringer International Publishing _c2011
300			_a1 recurso en línea (XIII, 153 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSynthesis Lectures on Synthetic Biology _x2151-0016
505	0		_aShort History of the use of Bacteria for Biosensing and Bioreporting -- Genetic Engineering Concepts -- Measuring with Bioreporters -- Epilogue.
520			_aBacterial reporters are live, genetically engineered cells with promising application in bioanalytics. They contain genetic circuitry to produce a cellular sensing element, which detects the target compound and relays the detection to specific synthesis of so-called reporter proteins (the presence or activity of which is easy to quantify). Bioassays with bacterial reporters are a useful complement to chemical analytics because they measure biological responses rather than total chemical concentrations. Simple bacterial reporter assays may also replace more costly chemical methods as a first line sample analysis technique. Recent promising developments integrate bacterial reporter cells with microsystems to produce bacterial biosensors. This lecture presents an in-depth treatment of the synthetic biological design principles of bacterial reporters, the engineering of which started as simple recombinant DNA puzzles, but has now become a more rational approach of choosing and combining sensing, controlling and reporting DNA 'parts'. Several examples of existing bacterial reporter designs and their genetic circuitry will be illustrated. Besides the design principles, the lecture also focuses on the application principles of bacterial reporter assays. A variety of assay formats will be illustrated, and principles of quantification will be dealt with. In addition to this discussion, substantial reference material is supplied in various Annexes. Table of Contents: Short History of the use of Bacteria for Biosensing and Bioreporting / Genetic Engineering Concepts / Measuring with Bioreporters / Epilogue.
988			_aSynthesis Collection of Technology_2011
650		7	_2embne _9158891 _aBiosensores
650		7	_2embne _9156012 _aMarcadores genéticos
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9783031014420
776	0	8	_iPrinted edition: _z9783031036989
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-031-02570-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b03/2023 _dz _esc _zSI