000			03928nam a22004575i 4500
999			_c387174 _d387174
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003			ES-MaUEC
005			20230212112636.0
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007			cr nn 008mamaa
008			220601s2020 sz | s |||| 0|eng d
020			_a9783031016660
024	7		_a10.1007/978-3-031-01666-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP519.9.E434 _b2020 EB
100	1		_aHuang, Liang _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_a3D Electro-Rotation of Single Cells _cby Liang Huang, Guido Buonincontri, Wenhui Wang
250			_a1st edition 2020
264		1	_aCham _bSpringer International Publishing _c2020
300			_a1 recurso en línea (XVII, 101 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSynthesis Lectures on Biomedical Engineering _x1930-0336
505	0		_aAcknowledgments -- Introduction -- Thick-Electrode DEP for Single-Cell 3D Rotation -- Opto-Electronic Integration of Thick-Electrode DEP Microfluidic Chip -- Summary and Outlook -- References -- Authors' Biographies.
520			_aDielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.
988			_aSynthesis Collection of Technology_2020
650		7	_2embne _9139103 _aBiología molecular
650		7	_2embne _9301792 _aCitología _xTécnica
650		7	_2embne _9141793 _aElectroforesis
700	1		_aBuonincontri, Guido _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9686216
700	1		_aWang, Wenhui _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9686776 _c(Of Tsinghua University)
776	0	8	_iPrinted edition: _z9783031000454
776	0	8	_iPrinted edition: _z9783031005381
776	0	8	_iPrinted edition: _z9783031027949
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-031-01666-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2023 _dz _esc _zSI