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008			220125s2022 si | s |||| 0|eng d
020			_a9789811649875
024	7		_a10.1007/978-981-16-4987-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.2 _b2022 EB
245	1	0	_aTextbook on Cloning, Expression and Purification of Recombinant Proteins _cedited by Kakoli Bose.
250			_aFirst edition 2022
264		1	_aSingapore _bSpringer International Publishing _c2022
300			_a1 recurso en línea (XII, 311 páginas) _b1 ilustraciones
336			_2rdacontent _aTexto _btxt
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
347			_aarchivo de texto _bPDF
505	0		_aA Brief Introduction to Recombinant DNA Technology -- Cloning and Gene Manipulation -- Selection of Cloning and Expression Plasmid Vectors -- Transformation and Protein Expression -- Introduction to Recombinant Protein Purification -- Protein Purification by Affinity Chromatography -- Protein Purification by Ion Exchange Chromatography -- Gel Filtration Chromatography -- Protein Purification by Reversed Phase Chromatography and Hydrophobic Interaction Chromatography -- Purification of Difficult Proteins -- Protein Quantitation and Detection. .
520			_aThis book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
988			_aSpringer_BiomedLife_2022
650		7	_2embne _9140931 _aBiotecnología
700			_aBose, Kakoli _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt _993873
776	0	8	_iPrinted edition: _z9789811649868
776	0	8	_iPrinted edition: _z9789811649882
776	0	8	_iPrinted edition: _z9789811649899
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-981-16-4987-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE _n0
998			_b04/2022 _dz _ep _zSI