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008			210915s2021 si | s |||| 0|eng d
020			_a9789811615368
024	7		_a10.1007/978-981-16-1536-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2021 EB
245	0	0	_aMethods for Fragments Screening Using Surface Plasmon Resonance _cedited by Sameer Mahmood Zaheer, Ramachandraiah Gosu
250			_aFirst edition 2021
264		1	_aSingapore _bSpringer International Publishing _c2021
300			_a1 recurso en línea (XVI, 113 páginas) _b52 ilustraciones, 37 ilustraciones a color
336			_2rdacontent _aTexto _btxt
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
347			_aArchivo de texto _bPDF
490	0		_aSpringer Protocols (Springer-12345)
505	0		_aChapter 1. Introduction to Surface Plasmon Resonance -- Chapter 2. Principle of Surface Plasmon Resonance (OneStep) -- Chapter 3. Preparation of protein with Histidine Tag for Amine coupling -- Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture -- Chapter 5. Protein stability using Thermal Shift Assay (TSA) - pH Tolerance -- Chapter 6. Protein stability using Thermal Shift Assay (TSA) - DMSO Tolerance -- Chapter 7. Selection and Identification of Fragment library -- Chapter 8. Preparation of Sensor -- Chapter 9. Optimization and validation of Amine coupling of Ligand -- Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand -- Chapter 11. Preparation of Fragments for Screening in SPR -- Chapter 12. Screening of Fragments in SPR -- Chapter 13. Identification of Fragment Hits -- Chapter 14. Data Analysis and Confirmation of Hits.
520	3		_aThis volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
988			_aSpringer_BiomedLife_2021
650		7	_2embne _aProteínas _9139861
650		7	_2embne _9158891 _aBiosensores
700	1		_aZaheer, Sameer Mahmood. _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aGosu, Ramachandraiah. _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9789811615351
776	0	8	_iPrinted edition: _z9789811615375
776	0	8	_iPrinted edition: _z9789811615382
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-981-16-1536-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b02/2022 _dz _eb _zSI