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710	2		_aSpringerLink (Online service) _9106996
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020			_a9789811380945
024	7		_a10.1007/978-981-13-8094-5 _2doi
040			_bspa _dES-MaUEC _cES-MaUEC
050		4	_aRB43.8 .D62 _b2019 EB
100	1		_aSong, Seo Woo _eautor _9670811
245	1	0	_aOne-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays _cby Seo Woo Song
264		1	_aSingapore _bSpringer Singapore : _bImprint: Springer _c2019
300			_a1 recurso en línea (XXI, 52 páginas) _b46 ilustraciones, 40 ilustraciones a color
336			_2rdacontent _aTexto _btxt
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
347			_atext file _bPDF
490	0		_aEngineering (Springer-11647)
490	0		_aSpringer Theses Recognizing Outstanding Ph.D. Research _x2190-5053
505	0		_aAbstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the 'Pipetting-Free' HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography.
520	3		_aThis thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients' primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of 'partipetting' for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications.
650		7	_2embne _aMicromatrices de ADN _9670812
650		7	_2embne _aTecnología médica _9150466
776	0	8	_iPrinted edition: _z9789811380938
776	0	8	_iPrinted edition: _z9789811380952
776	0	8	_iPrinted edition: _z9789811380969
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-981-13-8094-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aPrimersemestre_2019_Engineering
998			_aSI _cm _dz _feng _ggw _h0 _b09/2019 _eel _zSI