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336			_aTexto _btxt _2rdacontent
988			_aSpringerBiomedLife_2019
999			_c109241 _d109241 _x1
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005			20230110040216.0
008			190417s2019 ne | o |||| 0|eng d
020			_a9789402416626
024	7		_a10.1007/978-94-024-1662-6 _2doi
050		4	_aQH442.2 _b2019 EB
100	1		_aChoi, Seok-Yong _eautor _9670022
245	1	0	_aDNA Cloning: A Hands-on Approach _cby Seok-Yong Choi, Hyunju Ro, Hankuil Yi.
264		1	_aDordrecht _bSpringer International Publishing _c2019
300			_a1 recurso en línea (XIII, 131 páginas) _b93 ilustraciones, 70 ilustraciones a color
337			_aelectrónico _bc
338			_arecurso electrónico _bcr
347			_atext file _bPDF
490	0		_aBiomedical and Life Sciences (Springer-11642)
505	0		_aPart I. What is cloning? -- Chapter 1.1 Definition of cloning -- Chapter 1.2 Discovering a new gene -- Chapter 1.3 Cloning in the past -- Chapter 1.4 Cloning in the present -- Part II. A prerequisite for cloning -- Chapter 2.1 Software useful for cloning design -- Chapter 2.2 Vector, plasmid, construct and the Kozak consensus sequence -- Chapter 2.3 Multiple cloning sites (MCS) -- Chapter 2.4 Restriction endonucleases and star activity -- Chapter 2.5 Agarose gel electrophoresis -- Chapter 2.6 Pouring LB agar plates -- Chapter 2.7 Competent cells -- Chapter 2.8 The conversion of DNA mass into molar concentration -- Chapter 2.9 Upon receiving new plasmids -- Chapter 2.10 cDNA library -- Part III. The first step in cloning -- Chapter 3.1 Cut & paste -- Chapter 3.2 DNA sequencing and direct sequencing -- Chapter 3.3 PCR and nested PCR -- Chapter 3.4 Fill-in (Full & Partial) -- Chapter 3.5 Compatible cohesive ends -- Chapter 3.6 Methylation -- Chapter 3.7 Three-piece ligation -- Chapter 3.8 Site-directed mutagenesis -- Chapter 3.9 Structure of plant transformation vectors -- Chapter 3.10 Transformation of R. radiobacter -- Part IV. The next step of cloning -- Chapter 4.1 How to insert a DNA fragment into a gene -- Chapter 4.2 How to delete an internal region of a gene -- Chapter 4.3 How to insert an epitope tag into a gene -- Chapter 4.4 Translational fusion vs. transcriptional fusion -- Part V. The last steps of cloning -- Chapter 5.1 Method for cloning similar genes in different species -- Chapter 5.2 RACE (rapid amplification of cDNA ends) -- Chapter 5.3 BAC recombineering -- Chapter 5.4 Old trick: partial digestion -- Chapter 5.5 Modification of a vector -- Chapter 5.6 When you notice a frame shift mutation upon cloning -- Chapter 5.7 Reality of cloning: an extremely unlucky case -- Part VI. Methods that make your cloning life easier -- Chapter 6.1 TA cloning and production of a T-vector -- Chapter 6.2 TOPO TA cloning -- Chapter 6.3 Gateway cloning -- Chapter 6.4 Golden Gate Assembly for a modular cloning -- Chapter 6.5 In-Fusion Sequence and Ligation-Independent Cloning (In-Fusion SLIC) -- Chapter 6.6 T4 DNA Polymerase Sequence-and Ligation-Independent Cloning (T4 DNA Pol SLIC) -- Chapter 6.7 Non-template PCR cloning -- Part VII. Advice to cloners -- Chapter 7.1 When cloning is not going well -- Chapter 7.2 Keep your cloning data organized -- Appendix 1. Further readings -- Appendix 2. Abbreviations -- Appendix 3. Index.
520	3		_aThis book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail. .
650		7	_2embne _9159969 _aClonación molecular
650		7	_2embne _9162770 _aExpresión génica
653		0	_aGenetic engineering.
700	1		_aRo, Hyunju. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
700	1		_aYi, Hankuil. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
776	0	8	_iPrinted edition: _z9789402416602
776	0	8	_iPrinted edition: _z9789402416619
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi-org/10.1007/978-94-024-1662-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_aSI _cm _dz _feng _ggw _h0 _b07/2019 _ek _zSI