000			03992nam a22004335i 4500
710	2		_aSpringerLink (Online service) _0http://id.loc.gov/authorities/names/no2005046756 _1http://viaf.org/viaf/148105729 _9106996
999			_c108077 _d108077 _x1
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007			cr nn 008mamaa
008			171230s2018 si | s |||| 0|eng d
020			_a9789811067662
024	7		_a10.1007/978-981-10-6766-2 _2doi
040			_aES-MaUEC _bspa _dES-MaUEC
050		4	_aQP519.9 _b2018 EB
100	1		_aHosseini, Samira _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _0http://id.loc.gov/authorities/names/n2016183052 _1http://viaf.org/viaf/43153182676626791527 _9100738
245	1	0	_aEnzyme-linked Immunosorbent Assay (ELISA) : _bFrom A to Z _cby Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa
264		1	_aSingapore _bSpringer Singapore : _bImprint: Springer _c2018
300			_a1 recurso en línea (XI, 115 páginas) _b38 ilustraciones, 34 ilustraciones a color
336			_2rdacontent _aTexto (visual) _btxt
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
347			_atext file _bPDF _2rda
490	0		_aSpringerBriefs in Forensic and Medical Bioinformatic _x2196-8845
490	0		_aEngineering (Springer-11647)
505	0		_a1.Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISA -- 2.General overview on applications of ELISA -- 3.Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities -- 4. Evaluation of the detection results obtained from ELISA -- 5. Advantages, disadvantages and modifications of conventional ELISA -- 6.Common questions and answers about ELISA.
520			_aThis book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. .
650		7	_aEnzimas _2embne _9138640
650		7	_aBiotecnología _2embne _9140931
700	1		_aVázquez-Villegas, Patricia _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
700	1		_aRito-Palomares, Marco _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _1http://viaf.org/viaf/24153182730126791669
700	1		_aMartinez-Chapa, Sergio O _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _1http://viaf.org/viaf/837144647720246755354
776	0	8	_iPrinted edition: _z9789811067655
776	0	8	_iPrinted edition: _z9789811067679
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-981-10-6766-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b03/2019 _dz _ep _feng _ggw _h0