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040			_aES-MaUEC _bspa _cES-MaUEC
336			_aTexto _btxt _2rdacontent
710	2		_aSpringerLink (Online service) _0http://id.loc.gov/authorities/names/no2005046756 _1http://viaf.org/viaf/148105729 _9106996
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005			20230102113323.0
008			180526s2018 gw | s |||| 0|eng d
020			_a9783319779829
024	7		_a10.1007/978-3-319-77982-9 _2doi
050		4	_aQH442.2 .W66 _b2018 EB
100	1		_aWong, Dominic W. S _eautor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _0http://id.loc.gov/authorities/names/n88016841 _1http://viaf.org/viaf/76433502 _922769
245	1	4	_aThe ABCs of Gene Cloning _cby Dominic W. S. Wong.
250			_a3rd edition
264		1	_aCham, Switzerland _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIX, 257 páginas 172 ilustraciones,1 ilustraciones a color)
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
347			_atext file _bPDF
490	0		_aBiomedical and Life Sciences (Springer-11642)
505	0		_aFundamentals of Genetic Processes -- Introductory Concepts -- Structures of Nucleic Acids -- Structures of Proteins -- The Genetic Process -- Organization of Genes -- Reading The Nucleotide Sequence of a Gene -- Techniques and Strategies of Gene Cloning -- Enzymes Used in Cloning -- Techniques Used in Cloning -- Cloning Vectors for Introducing Genes into Host Cells -- Transformation -- Isolating Genes for Cloning -- Impact of Gene Cloning -- Improving Tomato Quality by Antisense Rna -- Transgenic Crops Engineered with Insecticidal Activity -- Transgenic Crops Conferred with Herbicide Resistance -- Growth Enhancement in Transgenic Fish -- Impact of Gene Cloning -- Microbial Production of Recombinant Human Insulin -- Finding Disease-Causing Genes -- Human Gene Therapy -- Gene Targeting -- DNA Typing -- Transpharmers - Bioreactors for Pharmaceutical Products -- Animal Cloning -- Human Genome Sequencing -- Manipulating Gene Vector Constructs.
520	3		_aClear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas. The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.
650		7	_aClonación molecular _9159969 _2embne
776	0	8	_iEdición impresa: _z9783319779836
776	0	8	_iEdición impresa: _z9783319777627
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-319-77982-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_aSI _b03/2019 _cm _dz _ea _feng _ggw _h0