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007			cr nn nnnaamaa
008			181108s2018 gw | s |||| 0|eng d
020			_a9783319980713
024	7		_a10.1007/978-3-319-98071-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
050		4	_aQH585.5.F56 _b2018 EB
100	1		_aGoetz, Christine _eautor _9669170
245	1	0	_aFlow Cytometry Basics for the Non-Expert _cby Christine Goetz, Christopher Hammerbeck, Jody Bonnevier
264		1	_aCham _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XVII, 219 páginas) _b117 ilustraciones, 113 ilustraciones a color
336			_aTexto _btxt _2rdacontent
347			_atext file _bPDF _2rda
490	0		_aTechniques in Life Science and Biomedicine for the Non-Expert _x2367-1114
490	0		_aBiomedical and Life Sciences (Springer-11642)
505	0		_a1. Flow Cytometry: Definition, History, and Uses in Biotechnology -- 2. Physics of a Flow Cytometer -- 3. The Language of Flow Cytometry and Experimental Setup -- 4. Fluorochrome Choices for Flow Cytometry -- 5. Compensation -- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry -- 7. Experimental Considerations with Data Sets as Examples -- 8. Cell Enrichment -- 9. Surface and Intracellular Staining Protocols for Flow Cytometry -- 10. Troubleshooting.
520	3		_aFlow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling in cells to detect the suppression of normal immune function, and analyzing biopsy specimens for tumors, to aid in cancer diagnosis and prognosis. In addition to simplifying the technique and using vocabulary accessible to those not trained in cell biology and immunology, the book includes the following 4 unqiue features: 1) Tips on how to set up an experiment for flow cytometry optimially 2) An expanded data set of flow staining including multiparameter flow cytometry 3) Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens) 4) Section devoted to antibody development and conjugation.
650		7	_aCitometría de flujo _2embne _9668358
700	1		_aHammerbeck, Christopher _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
700	1		_aBonnevier, Jody _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
710	2		_aSpringerLink (Online service) _0http://id.loc.gov/authorities/names/no2005046756 _9106996
776	0	8	_iPrinted edition: _z9783319980706
776	0	8	_iPrinted edition: _z9783319980720
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-319-98071-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc
988			_aEBSPRINGER_BIOMEDLIFE_2019
998			_aSI _a_alco _a_vill _b01/2019 _cm _dz _ef _feng _ggw _h0