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007			cr nn nnnaamaa
008			181111s2018 si | s |||| 0|eng d
020			_a9789811307850 _9
024	7		_a10.1007/978-981-13-0785-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
050		4	_aQP601 _b2018 EB
100	1		_aPunekar, N. S. _eautor _9669167
245	1	0	_aENZYMES : _bCatalysis, Kinetics and Mechanisms _cby N. S. Punekar
264		1	_aSingapore _bSpringer Singapore _c2018
300			_a1 recurso en línea (XXIII, 562 páginas) _b263 ilustraciones, 76 ilustraciones a color
336			_aTexto _btxt _2rdacontent
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
347			_atext file _bPDF _2rda
505	0		_aPart I: Enzyme catalysis - a perspective -- Enzymes -Their place in Biology -- Enzymes - Historical aspects -- Exploiting enzymes - Technology -- On enzyme nomenclature and classification -- Hallmarks of an enzyme catalyst -- Origins of enzyme catalytic power -- Origins of enzyme catalytic power -- Which enzyme uses what trick? Some remarks -- Structure and catalysis: Conformational flexibility and protein motion -- Part II: Enzyme kinetic practice and measurements -- Chemical kinetics - Fundamentals -- Concepts of equilibrium and steady-state -- ES complex and pre-steady-state kinetics -- Principles of enzyme assays -- Good kinetic practices -- Quantification of catalysis and measures of enzyme purity -- Henri-Michaelis-Menten equation -- More complex rate expressions -- Enzyme kinetic data - Collection and analysis -- Part III: Elucidation of Kinetic mechanisms -- Approaches to kinetic mechanism - Overview -- Analysis of initial velocity patterns -- Enzyme inhibition analyses -- Irreversible inhibitions -- Reversible inhibitions -- Alternate substrate (product) interactions -- pH Studies with enzymes -- Isotopes in enzymology -- Isotope exchanges at equilibrium -- Isotope effects in enzymology -- From kinetic data to mechanism and back -- Part IV: Chemical mechanisms and catalysis -- Chemical reactivity and molecular interactions -- Acid-base chemistry and catalysis -- Nucleophilic catalysis and covalent reaction intermediates -- Phosphoryl group chemistry and importance of ATP -- Enzymatic oxidation-reduction reactions -- Carboxylations and decarboxylations -- Electrophilic catalysis and amino acid transformations -- Integrating kinetic and chemical mechanisms - A synthesis -- Part V: Frontiers in enzymology -- Regulation of enzyme activity -- In vitro versus in vivo - Concept and consequences -- Future of enzymology - An appraisal -- Closure - Whither Enzymology.
520	3		_aThis enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to design enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.
650		7	_aEnzimas _2embne _9138640
650		7	_aEnzimología _9144680 _2embne
710	2		_aSpringerLink (Online service) _0http://id.loc.gov/authorities/names/no2005046756 _1http://viaf.org/viaf/148105729 _9106996
776	0	8	_iPrinted edition: _z9789811307843
776	0	8	_iPrinted edition: _z9789811307867
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-981-13-0785-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
490	0		_aBiomedical and Life Sciences (Springer-11642)
942			_2lcc
988			_aEBSPRINGER_BIOMEDLIFE_2019
998			_aSI _a_alco _a_vill _b01/2019 _cm _dz _ef _feng _ggw _h0
999			_c106828 _d106828 _x1