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008			150314s2015 gw | s |||| 0|eng d
020			_a9783319162539
024	7		_a10.1007/978-3-319-16253-9 _2doi
040			_bspa _dES-MaUEC
050		4	_aQP623.8.P75 _b2015 EB
100	1		_aMunschauer, Mathias. _eautor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _1http://viaf.org/viaf/309794405/
245	1	0	_aHigh-Resolution Profiling of Protein-RNA Interactions _cby Mathias Munschauer.
264		1	_aCham _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XXIII, 121 páginas 25 ilustraciones, 20 ilustraciones a color.)
336			_2rdacontent _aTexto (visual) _btxt
337			_2rdamedia _aelectrónico _bc
338			_2rdacarrier _arecurso electrónico _bcr
490	0		_aSpringer Theses, Recognizing Outstanding Ph.D. Research, _x2190-5053
490	0		_aEngineering (Springer-11647)
505	0		_aIntroduction -- Mapping Regulatory Interactions of the Rna-Binding Protein Lin28b -- Exploring The Sequence Space Contacted by the Ensemble of Rna-Binding Proteins -- Revealing Cell-Type Specific Differences in Protein Occupancy on Rna -- Discussion.
520	3		_aThe work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel 'protein occupancy profiling' technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3' UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universität Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.  .
988			_aEBSPRINGER_2018
650		7	_aGenética humana _2embne _9140562
650		7	_9140525 _aÁcidos nucleicos
776	0	8	_iEdición impresa: _z9783319162546
776	0	8	_iEdición impresa: _z9783319162522
776	0	8	_iEdición impresa: _z9783319368481
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-319-16253-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b03/2019 _dz _eIG _zSI