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008			180314s2018 si | s |||| 0|eng d
020			_a9789811075452 _9
024	7		_a10.1007/978-981-10-7545-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
050		4	_aQH491 2018 EB
245	1	0	_aTransgenic Ascidians _cedited by Yasunori Sasakura.
264		1	_aSingapore _bSpringer International Publishing _c2018
300			_a1 recurso en línea (X, 201 páginas)
336			_aTexto _btxt _2rdacontent
347			_atext file _bPDF _2
490	0		_aAdvances in Experimental Medicine and Biology _x0065-2598 _v1029
520	3		_aThis book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.
650		7	_9162368 _aBiología del desarrollo _2embne
650		7	_aCitología _2embne _9139477
700	1		_aSasakura, Yasunori _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt _0http://id.loc.gov/authorities/names/no2018087382 _1http://viaf.org/viaf/61153124328224492121
710	2		_aSpringerLink (Online service) _0http://id.loc.gov/authorities/names/no2005046756 _1http://viaf.org/viaf/148105729 _9106996
776	0	8	_iEdición impresa: _z9789811075445
776	0	8	_iEdición impresa: _z9789811075469
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-981-10-7545-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
490	0		_aBiomedical and Life Sciences (Springer-11642)
942			_2lcc
998			_aSI _b03/2019 _cm _dz _ep _feng _ggw _h0
999			_c100960 _d100960 _x1