A Guide to the Study of Plant Structure with Emphasis on Living Specimens -- Chemical and Physical Fixation of Cells and Tissues: an Overview -- Paraffin and Polyester Waxes -- The Glycol Methacrylate Embedding Resins â€{u0525}chnovit 7100 and 8100 -- Epoxy Resins for Light and Transmission Electron Microscopy -- LR White Acrylic Resin -- High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples -- Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens -- Fluorescent Staining of Living Plant Cells -- Improved Methods for Clearing and Staining of Plant Samples -- Whole Mount Immunofluorescence Staining of Plant Cells and Tissues -- Protoplast Isolation and Staining -- Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy.-Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving -- Abscisic Acid Immunostaining -- Plant Chromosome Preparations and Staining for Light Microscopic Studies -- Chromosome techniques and FISH -- Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2â€{uD925}oxyuridine (EdU) -- Staining Methods for Programmed Cell Death -- Laser Microdissection of Plant Tissues -- RNA In situ Hybridization -- Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy -- Collecting and Processing Wood Microcores for Monitoring Xylogenesis -- Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy -- Collection of Plant Remains from Archaeological Contexts -- Archaeological Wood Preparation -- Archaeopalynological Preparation Techniques -- Phytoliths: Preparation and Archaeological Extraction -- Starch Granules: Preparation and Archaeological Extraction -- Plant Collection, Identification, and Herbarium Procedures -- Index.

This work gathers common plant microtechniquesÂ{u1BA4} updates their procedures using a simple and fully understandable approach.The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time.This bookÂ{u1BA1}lyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume coversÂ{u4A25} more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled inÂ{u4A25} second sectionÂ{u4BE0}showcase the usefulness of various techniques based on different processing and staining methods.Â{u4A25} third sectionÂ{u8A67}hlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.