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A Practical Guide to Protein Engineering / Tuck Seng Wong, Kang Lan Tee.

By: Wong, Tuck Seng, author.
Contributor(s): Tee, Kang Lan, author. | SpringerLink
Material type: materialTypeLabelE-bookSeries: (Learning Materials in Biosciences, 2509-6125); (Biomedical and Life Sciences (SpringerNature-11642)); (Biomedical and Life Sciences (R0) (SpringerNature-43708)).Publisher: Cham : Springer International Publishing : Imprint: Springer, 2020Edition: First edition.Description: 1 recurso en línea (XV, 202 páginas) : 91 ilustraciones, 68 ilustraciones a color.ISBN: 9783030568986.Subject: Ingeniería biomédica | BiotecnologíaOnline resources: Acceso a este recurso digital (usuarios Universidad Europea de Madrid)Digital Resources
Contents:
Literature Search -- Sequence Analysis -- Structural Analysis -- Protein Expression Hosts and Expression Plasmids -- Gene Cloning -- Protein Expression -- Assay -- Gene Mutagenesis -- High-Throughput Screening (HTS) -- Protein Variants Analysis and Characterization -- Continuing from Protein Variants -- Employability.
In: Springer Nature eBookAbstract: This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
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Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
LIBRO-E NO PRÉSTAMO LIBRO-E NO PRÉSTAMO Madrid Digital Acceso Electrónico (UEM) Ciencias e Ingeniería TP248.65.P76 2020 EB (Browse shelf(Opens below)) Acceso electrónico eBook.15012047
Total holds: 0

Literature Search -- Sequence Analysis -- Structural Analysis -- Protein Expression Hosts and Expression Plasmids -- Gene Cloning -- Protein Expression -- Assay -- Gene Mutagenesis -- High-Throughput Screening (HTS) -- Protein Variants Analysis and Characterization -- Continuing from Protein Variants -- Employability.

This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

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