6.7 Future Prospects of Genome Editing in Plants.

Foreword; Contents; About the Authors; Part I: Introduction to the CRISPR Revolution; Chapter 1: Viral Vectors, Engineered Cells and the CRISPR Revolution; 1.1 Introduction; 1.2 CRISPR-Cas Genome Manipulation; 1.2.1 A Brief Overview of Genome Modification Using Endonucleases; 1.2.2 CRISPR-Cas Systems; 1.2.3 CRISPR Tools in Biology; 1.3 Gene Therapy Using Viruses; 1.3.1 Retroviral Vectors; 1.3.2 Modifications and Implementation of Retroviral Vectors; 1.3.3 Translational and Clinical Progress Using Retroviral Vectors; 1.4 Adenoviral Vectors.

1.4.1 Modifications and Implementation of Adenoviral Vectors1.4.2 Translational and Clinical Progress Using Adenoviral Vectors; 1.5 Adenoviral-Associated Viral Vectors; 1.5.1 Modifications and Implementation of Adeno-associated Viral Vectors; 1.5.2 Translational and Clinical Progress Using Adeno-Ưassociated Viral Vectors; 1.6 Ex Vivo CRISPR Therapies; 1.6.1 CAR T-Cell Therapy; 1.6.2 iPSCs; 1.7 Conclusion; References; Chapter 2: Combining Engineered Nucleases with Adeno-Ưassociated Viral Vectors for Therapeutic Gene Editing; 2.1 Introduction; 2.2 Therapeutic Gene Editing; 2.2.1 AAV.

2.2.2 Nucleases and AAV for Therapeutic Gene Editing2.2.3 Challenges; 2.3 Conclusion; References; Part II: CRISPR in Model Systems; Chapter 3: From Reductionism to Holism: Toward a More Complete View of Development Through Genome Engineering; 3.1 Introduction and Historical Context; 3.2 CRISPR Genome Editing in Brief; 3.3 A Genomics Perspective; 3.3.1 Gene Network Analysis with CRISPR GE; 3.3.2 Mapping and Understanding Regulatory DNA Within the Genomic Context with CRISPR GE; 3.4 An Epigenomics Perspective; 3.4.1 Manipulating DNA and Histone Modifications with CRISPR GE.

3.4.2 Tracking 3D Genomic Structure with CRISPR GE3.5 A Cellular Perspective; 3.5.1 Lineage Tracing with CRISPR GE; 3.5.2 CRISPR GE and Ex Vivo Organogenesis; 3.5.3 Final Thoughts; References; Chapter 4: A Transgenic Core Facility#x80;#x99;s Experience in Genome Editing Revolution; 4.1 Pronuclear Microinjection; 4.2 ES Cell Injection; 4.3 Cytoplasmic Microinjection; 4.4 A New Era of Animal Model Production by the CRISPR/Cas9 Technology; 4.5 Guide RNA Activity is the Key; 4.6 Design of the CRISPR/Cas9-Mediated Targeting; 4.7 Conclusion and Perspectives; References.

Chapter 5: Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors5.1 Introduction; 5.2 Ca2+ Homeostasis in Photoreceptors; 5.3 Genome Editing in Zebrafish; References; Chapter 6: CRISPR: From Prokaryotic Immune Systems to Plant Genome Editing Tools; 6.1 Introduction; 6.2 What Is CRISPR?; 6.3 History of CRISPR; 6.4 Cas9 and Cpf1: The Lead Players in CRISPR-Based Genome Editing; 6.5 Modification of the CRISPR System in Plants; 6.6 Application of CRISPR in Plants; 6.6.1 Model Plants; 6.6.2 Application in Crop Plants; 6.6.3 Off-Target Effects.

This book presents descriptive overviews of gene editing strategies across multiple species while also offering in-depth insight on complex cases of application in the field of tissue engineering and regenerative medicine.  Chapters feature contributions from leaders in stem cell therapy and biology, providing a comprehensive view of the application of gene therapy in numerous fields with an emphasis on ophthalmology, stem cells, and agriculture. The book also highlights recent major technological advances, including ZFN, TALEN, and CRISPR. Precision Medicine, CRISPR, and Genome Engineering is part of the highly successful Advances in Experimental Medicine and Biology series. It is an indispensable resource for researchers and students in genetics as well as clinicians. .

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