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Multi-parametric live cell microscopy of 3D tissue models / Ruslan I. Dmitriev, editor.

Contributor(s): Dmitriev, Ruslan I.,, editor literario
Material type: materialTypeLabelE-bookSeries: (Advances in experimental medicine and biology, 0065-2598).Publisher: Cham, Switzerland : Springer International Publishing, 2017Description: 1 recurso en línea : ilustraciones (algunas a color).ISBN: 3319673580; 9783319673585.Subject: Ingeniería biomédicaOnline resources: Acceso a este recurso digital (usuarios Universidad Europea de Madrid)Digital Resources
Contents:
""Preface""; ""Contents""; ""Contributors""; ""Part I: Introduction: 3D Tissue Models, Methodology and Toolkit""; ""1: Current State-of-the-Art 3D Tissue Models and Their Compatibility with Live Cell Imaging""; ""1.1 Introduction""; ""1.2 Types of 3D Tissue Models""; ""1.2.1 Scaffold-Free Cultures""; ""1.2.2 Scaffold-Cell Constructs""; ""1.3 Imaging Modalities for Live Cells""; ""1.3.1 Confocal Laser Scanning Microscopy and Epifluorescence Microscopy""; ""1.3.2 Fluorescence Lifetime Imaging (FLIM) and Phosphorescence Lifetime Imaging (PLIM)""
""1.3.3 Optical Coherence Tomography""""1.3.4 Micro-Computerised Tomography""; ""1.3.5 Indirect Live Cell Imaging""; ""1.3.5.1 Monitoring Scaffold Degradation""; ""1.3.5.2 Monitoring Extracellular Matrix Deposition by Live Cells""; ""1.4 Advantages and Challenges of Live Cell Imaging""; ""1.5 Prospect and Summary""; ""References""; ""2: Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation""; ""2.1 Motivation of Using Phosphorescence Lifetime Imaging""; ""2.2 Technical Challenges""
""2.2.1 Excitation Pulse Period and Laser Power""""2.2.2 Pile-Up Effect""; ""2.2.3 Detector Overload""; ""2.2.4 Interference with Scanning""; ""2.3 FLIMâ#x80;#x94;PLIM by Multipulse Excitation""; ""2.4 Implementation in Laser Scanning Systems""; ""2.4.1 DCS-120 Confocal Scanning FLIM System""; ""2.4.2 Zeiss LSM 710, 780, 880 Systems""; ""2.4.3 Leica SP Multiphoton Systems""; ""2.4.4 Sutter Instrument MOM Microscopes""; ""2.5 Applications""; ""2.5.1 Oxygen Sensing""; ""2.5.2 Simultaneous Recording of pO2 and NAD(P)H Images""; ""2.5.3 Detection of Zinc Oxide Nanoparticles""
""2.5.4 PLIM of Inorganic Materials""""2.6 Suppression of Autofluorescence""; ""2.7 Summary""; ""References""; ""3: Quantitative Live Cell FLIM Imaging in Three Dimensions""; ""3.1 Introduction""; ""3.2 Implementations of Fluorescence Lifetime Imaging""; ""3.2.1 Time-Domain FLIM Systems""; ""3.2.1.1 Time-Correlated Single Photon Counting (TCSPC)""; ""Confocal TCSPC-FLIM""; ""Multi-Photon TCSPC-FLIM""; ""3.2.1.2 Time-Gated systems""; ""3.2.2 Frequency Domain systems""; ""3.3 Multi-Channel FLIM""; ""3.3.1 Multi-Color and Spectrally-­Resolved FLIM""
""3.3.2 Polarization-Resolved FLIM""""3.4 FLIM in Combination with Other Microscopy Techniques""; ""3.4.1 Combined FLIM and PLIM""; ""3.4.2 FLIM and Optical Coherence Tomography (OCT)""; ""3.4.3 FLIM with Nonlinear Microscopy Techniques for Label-Free Imaging of Complex Tissue""; ""3.4.4 FLIM for Clinical Imaging""; ""3.4.5 Perspective: Adaptive Optics for Improved Optical Sectioning in High Resolution Microscopy""; ""3.5 Analysis of FLIM Data""; ""3.5.1 Time-Domain Data""; ""3.5.1.1 Pixel-Wise Decay Fitting""; ""3.5.1.2 Global Fitting Techniques""
Abstract: This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
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Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
LIBRO-E NO PRÉSTAMO LIBRO-E NO PRÉSTAMO Madrid Digital Acceso Electrónico (UEM) Ciencias de la Salud R857.T55 .D65 2017 EB (Browse shelf(Opens below)) Acceso electrónico eBook.20024252
Total holds: 0

""Preface""; ""Contents""; ""Contributors""; ""Part I: Introduction: 3D Tissue Models, Methodology and Toolkit""; ""1: Current State-of-the-Art 3D Tissue Models and Their Compatibility with Live Cell Imaging""; ""1.1 Introduction""; ""1.2 Types of 3D Tissue Models""; ""1.2.1 Scaffold-Free Cultures""; ""1.2.2 Scaffold-Cell Constructs""; ""1.3 Imaging Modalities for Live Cells""; ""1.3.1 Confocal Laser Scanning Microscopy and Epifluorescence Microscopy""; ""1.3.2 Fluorescence Lifetime Imaging (FLIM) and Phosphorescence Lifetime Imaging (PLIM)""

""1.3.3 Optical Coherence Tomography""""1.3.4 Micro-Computerised Tomography""; ""1.3.5 Indirect Live Cell Imaging""; ""1.3.5.1 Monitoring Scaffold Degradation""; ""1.3.5.2 Monitoring Extracellular Matrix Deposition by Live Cells""; ""1.4 Advantages and Challenges of Live Cell Imaging""; ""1.5 Prospect and Summary""; ""References""; ""2: Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation""; ""2.1 Motivation of Using Phosphorescence Lifetime Imaging""; ""2.2 Technical Challenges""

""2.2.1 Excitation Pulse Period and Laser Power""""2.2.2 Pile-Up Effect""; ""2.2.3 Detector Overload""; ""2.2.4 Interference with Scanning""; ""2.3 FLIMâ#x80;#x94;PLIM by Multipulse Excitation""; ""2.4 Implementation in Laser Scanning Systems""; ""2.4.1 DCS-120 Confocal Scanning FLIM System""; ""2.4.2 Zeiss LSM 710, 780, 880 Systems""; ""2.4.3 Leica SP Multiphoton Systems""; ""2.4.4 Sutter Instrument MOM Microscopes""; ""2.5 Applications""; ""2.5.1 Oxygen Sensing""; ""2.5.2 Simultaneous Recording of pO2 and NAD(P)H Images""; ""2.5.3 Detection of Zinc Oxide Nanoparticles""

""2.5.4 PLIM of Inorganic Materials""""2.6 Suppression of Autofluorescence""; ""2.7 Summary""; ""References""; ""3: Quantitative Live Cell FLIM Imaging in Three Dimensions""; ""3.1 Introduction""; ""3.2 Implementations of Fluorescence Lifetime Imaging""; ""3.2.1 Time-Domain FLIM Systems""; ""3.2.1.1 Time-Correlated Single Photon Counting (TCSPC)""; ""Confocal TCSPC-FLIM""; ""Multi-Photon TCSPC-FLIM""; ""3.2.1.2 Time-Gated systems""; ""3.2.2 Frequency Domain systems""; ""3.3 Multi-Channel FLIM""; ""3.3.1 Multi-Color and Spectrally-­Resolved FLIM""

""3.3.2 Polarization-Resolved FLIM""""3.4 FLIM in Combination with Other Microscopy Techniques""; ""3.4.1 Combined FLIM and PLIM""; ""3.4.2 FLIM and Optical Coherence Tomography (OCT)""; ""3.4.3 FLIM with Nonlinear Microscopy Techniques for Label-Free Imaging of Complex Tissue""; ""3.4.4 FLIM for Clinical Imaging""; ""3.4.5 Perspective: Adaptive Optics for Improved Optical Sectioning in High Resolution Microscopy""; ""3.5 Analysis of FLIM Data""; ""3.5.1 Time-Domain Data""; ""3.5.1.1 Pixel-Wise Decay Fitting""; ""3.5.1.2 Global Fitting Techniques""

This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.

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