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SpringerLink Springer Biomedical and Life Sciences eBooks 2017 English+International

Introduction; 1 Molecular Diagnostics of Bacterial Pathogens; Abstract; 1.1 Introduction; 1.2 Molecular Diagnostics of Bacterial Sexually Transmitted Infections; 1.2.1 Molecular Diagnostics of Chlamydia trachomatis and Neisseria gonorrhoeae; 1.2.2 Molecular Diagnostics of Other Sexually Transmitted Bacteria; 1.3 Molecular Diagnostics of Bacterial Gastroenteritis; 1.3.1 Major Bacterial Pathogens for Routine Microbiological Diagnostics; 1.3.2 Other Bacterial Pathogens; 1.3.3 Molecular Diagnostics Algorithms for the Identification of Enteric Pathogens; 1.4 Bacterial Typing.

1.4.1 Typing Methods1.4.2 Pulsed-Field Gel Electrophoresis (PFGE); 1.4.3 Amplified Fragment Length Polymorphism Typing (AFLP); 1.4.4 Variable Number of Tandem Repeat (VNTR) Typing; 1.4.5 Random Amplification of Polymorphic DNA (RAPD); 1.4.6 Other Amplification Based Methods; 1.4.7 Single Locus Sequence Typing; 1.4.8 Spa Typing; 1.4.9 Multilocus Sequence Typing; 1.4.10 Optical Mapping; 1.4.11 Whole Genome Sequencing (WGS); 1.4.12 Typing for Action; 1.5 Conclusion; Recommended Literature; 2 Molecular Diagnostics of Parasitic Infections; Abstract; 2.1 Introduction; 2.2 DNA Isolation.

2.3 Intestinal Parasites2.3.1 Protozoa; 2.3.2 Helminths (Worms); 2.3.3 Algorithm for Faecal Examination; 2.4 Blood and Tissue Parasites; 2.4.1 Protozoa; 2.4.2 Helminths; 2.5 Concluding Remarks; References and Recommended Literature; 3 Virology; Abstract; 3.1 Introduction; 3.2 Isolation of Viral Nucleic Acids from Clinical Samples; 3.3 Molecular Diagnostics of Viruses; 3.3.1 Viral Detection Methods; 3.3.2 Primer/Probe Design; 3.3.2.1 Mismatch-Tolerance in Primers; 3.3.2.2 Probe Design; 3.4 Molecular Techniques for the Detection of Viral Variation.

3.5 Clinical Applications of Molecular Diagnostics3.5.1 Molecular Detection of Viral Infections; 3.5.2 Quantitative Detection of Viruses-Viral Load; 3.5.3 Genotyping and Genotypic Determination of Viral Resistance; 3.5.3.1 HIV-1; 3.5.3.2 Hepatitis B; 3.5.3.3 Hepatitis C; 3.5.3.4 Herpes Viruses; 3.5.3.5 Influenza Virus; 3.6 Conclusions; Recommended Literature; 4 Mycology; Abstract; 4.1 Introduction; 4.2 Molecular detection of invasive fungal infections; 4.2.1 Invasive Fungal Infections; 4.2.1.1 Diagnosis of Invasive Fungal Infection; 4.2.1.2 Conventional Species Identification.

4.2.1.3 Molecular Detection Methods4.2.1.4 Choice of Specimen; 4.2.1.5 Isolation of Fungal DNA; 4.2.2 Invasive Candidiasis; 4.2.2.1 Molecular Detection of Invasive Candidiasis; 4.2.3 Invasive Aspergillosis; 4.2.3.1 Molecular Detection; 4.2.4 Mucorales, Scedosporium, Fusarium and Other Opportunistic Filamentous Moulds; 4.2.4.1 Molecular Detection; 4.2.5 Pneumocystis jiroveci Pneumonia; 4.2.5.1 Molecular Identification; 4.3 Molecular diagnostics of dermatophyte infections; 4.3.1 Introduction; 4.3.2 Dermatophytosis; 4.3.3 Taxonomy and Epidemiology of Dermatophytes.

This two volume book set provides a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are currently used in a wide variety of disciplines. The volumes cover: Clinical microbiology and virology Clinical chemistry Pathology Veterinary medicine Plant Pathology Food safety The two volumes are written by experts in their respective fields, who have, together with the editors, combined years of experience in the development, application and quality control of molecular diagnostic methods. The first book is devoted to the theory and backgrounds of molecular techniques, amplification technology, next generation sequencing and bioinformatics for molecular laboratory diagnostics. As a fundament of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is extensively discussed. The second book highlights the applications of these methods in the various diagnostic laboratories. These two full-colour well-illustrated volumes are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic tests and for those who wish to expand their knowledge on the current molecular biological revolution. The extensive information in both books highlights the current trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.