Incluye referencias bibliográficas

Foreword; Reference ; Contents; About the Editors; Contributors; Chapter 1: Overview and Sources of Reactive Oxygen Species (ROS) in the Reproductive System; 1.1 Introduction; 1.1.1 Free Radicals; 1.1.1.1 Superoxide Anion (O2−); 1.1.1.2 Hydroxyl Radical (OH); 1.1.2 Oxidation-Reduction (Redox) Reactions; 1.1.2.1 Haber-Weiss Reaction; 1.1.2.2 Electron Transport Chain (ETC); 1.1.3 Reactive Oxygen Species (ROS); 1.1.3.1 Physiological Roles; 1.1.4 Reactive Nitrogen Species (RNS); 1.1.4.1 Physiological Roles; 1.1.5 Sources of ROS in Male Reproductive System; 1.1.5.1 Generation of ROS

2.1 Introduction2.1.1 Types of Semen Sample Used to Detect ROS; 2.1.2 Measurement of ROS; 2.1.2.1 Nitroblue Tetrazolium Test; 2.1.2.2 Chemiluminescence Assay; 2.1.3 Measurement of DNA Fragmentation; 2.1.3.1 Sperm Chromatin Structure Assay (SCSA); 2.1.3.2 Terminal deoxynucleotidyl Transferees dUTP Nick End Labeling (TUNEL) Assay; 2.1.3.3 Epifluorescence Using Acridine Orange Dye; 2.1.3.4 Comet Assay; 2.1.3.5 Sperm Chromatin Dispersion (SCD) Assay; 2.1.4 Other Methods for Measuring ROS; 2.1.4.1 Measurement of Total Antioxidant Capacity; Colorimetric Analysis; Oxygen Radical Absorbance

3.1 Introduction3.1.1 Physiological Roles of ROS in Male Reproductive System; 3.1.1.1 Spermatogenesis and Sperm Maturation; 3.1.1.2 Sperm Capacitation; 3.1.1.3 Acrosome Reaction; 3.1.1.4 Sperm-oocyte Fusion; 3.2 Conclusion; 3.2.1 Physiological Roles of ROS in the Female Reproductive System; 3.2.1.1 Follicular Development; 3.2.1.2 Ovarian Steroidogenesis; 3.2.1.3 Ovulation; 3.2.1.4 Corpus Luteum; Formation; Luteolysis; 3.2.1.5 Maintenance of Pregnancy; 3.2.1.6 Parturition; 3.3 Conclusion; References; Chapter 4: Negative Effects of Oxidative Stress (OS) on Reproductive System at Cellular Level

Ferric Reducing Ability Assay2.1.4.2 ROS-TAC Score; 2.1.4.3 Measurement of Redox Potential; 2.1.5 Measurement of Lipid Peroxidation; 2.1.5.1 HNE-His Adduct ELISA/HNE Adduct Competitive ELISA; 2.1.5.2 Isprostane (IsoP) Method; 2.1.5.3 TBARS Assay; 2.1.6 Measurement of ROS-Induced Post-Translational Modifications; 2.1.7 Measurement of ROS-Induced Protein Alterations-Proteomic Analysis; 2.1.7.1 Validation of Proteins of Interest; Western Blot Analysis; Immunochemistry; ELISA; 2.2 Conclusion; References; Chapter 3: Physiological Roles of Reactive Oxygen Species (ROS) in the Reproductive System

NADPH OxidasesElectron Leakage in the Mitochondria; 1.1.5.2 Sources of ROS in Seminal Plasma; Immature/Abnormal Spermatozoa; Leukocytes; Varicocele; 1.2 Conclusion; 1.2.1 Sources of ROS in Female Reproductive System; 1.2.2 Steroidogenesis and Ovulation; 1.2.3 Graffian Follicle; 1.2.4 Granulosa Cells and Cumulus Mass Cells; 1.2.5 Follicular Fluid; 1.2.6 Fallopian Tube; 1.2.7 Peritoneal Cavity; 1.2.8 Endometrium; 1.3 Conclusion; 1.3.1 Oxidative Stress (OS); References; Chapter 2: Methods to Measure Reactive Oxygen Species (ROS) and Total Antioxidant Capacity (TAC) in the Reproductive System

This book discusses the role of oxidative stress in the reproductive system. The book reviews endogenous sources, methods of determining its levels in body fluid/tissues, the physiological roles of ROS, as well as its negative effects on the human reproductive processes. Also discussed are multiple extrinsic factors that could induce oxidative stress in the reproductive system. This brief covers various clinical pathologies related to the reproductive system that arise from or produce oxidative stress, both in the male and female. The use of antioxidants as a therapeutic measure to keep ROS levels in check are highlighted, describing the outcome of various clinical studies involving antioxidant supplementation in infertile patients. Infertility is a global disease that affects 15-25% of all couples, and oxidative stress arising from a multitude of sources has been implicated as one of the major contributing factors to the decline in human fertility. As such, this book provides an up-to-date review on the significance of ROS in human reproduction.