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Preface; Contents; 1 Fluorescence Made Easier: Fluorescence Techniques for the Novice. Episode 1: The Basics; 1.1 Basic Concepts, with a Minimum of Annoying Physics: The Golden Rules; 1.1.1 Golden Rule 1: The Fluorescence Is Always at Longer Wavelengths than the Excitation; 1.1.2 Golden Rule 2: The Shape of the Emission Spectrum Does Not Change as the Excitation Wavelength Is Varied; 1.1.2.1 Quantum Yield; 1.1.2.2 Measuring Fluorescence Intensities and Spectra; 1.1.3 Golden Rule 3: Ordinarily, the Excitation Spectrum Is the Same Shape as the Absorption Spectrum; Glossary.

2 Fluorescence Made Easier: Fluorescence Techniques for the Novice. Episode 2: Using Fluorescence Anisotropy or Polarization to View Intermolecular Associations2.1 What's Behind Fluorescence Anisotropy?; 2.2 To Understand Fluorescence Anisotropy or Polarization, You Need to Know One Simple Equation; 2.3 To Measure Fluorescence Anisotropy or Polarization, You Need to Think About Light; References; 3 Time-Gated FRET Detection for Multiplexed Biosensing; 3.1 Introduction; 3.2 Theory of Time-Gated (TG) FRET; 3.2.1 Time-Gated vs. Time-Resolved Luminescence.

3.2.2 TG FRET (Förster Resonance Energy Transfer)3.2.3 Multiplexed TG FRET; 3.3 Advantages of TG FRET; 3.3.1 Single Analyte Detection; 3.3.2 Multiplexed Detection; 3.4 Spectroscopy-Based TG FRET Biosensing; 3.4.1 Equipment and Detection Formats; 3.4.2 Single Analyte Biosensing Applications; 3.4.3 Multiplexed Biosensing Applications; 3.5 Microscopy-Based TG FRET Biosensing; 3.5.1 Equipment and Detection Formats; 3.5.2 TG Luminescence Biosensing; 3.5.3 TG FRET Biosensing; 3.6 Conclusions and Future Perspectives; References.

4 Pharmacologically Active Plant Flavonols as Proton Transfer Based Multiparametric Fluorescence Probes Targeting Biomolecules: Perspectives and Prospects4.1 Introduction: Background and Historical Perspectives; 4.1.1 Fluorescence as an Exquisitely Sensitive Approach for Exploring Biomolecular Interactions: A Brief Overview; 4.1.2 Plant Flavonoids: Natural Distribution, Chemical Classification, Therapeutic Importance, and Intrinsic Fluorescence Behavior.

4.1.3 Excited-State Intramolecular Proton Transfer (ESIPT) and `Two Color' Fluorescence of Flavonols: Historical Perspective and Sensitivity to Environment4.2 Applications of ESIPT and `Two Color' Fluorescence of Therapeutically Active Flavonols with Representative Bio-relevant Targets: Scope of the Present Survey and Fluorescence Methodologies; 4.3 Protein-Flavonol Interactions; 4.3.1 Background; 4.3.2 Serum Albumin-Flavonol Interactions; 4.3.3 Hemoglobin-Flavonol Interaction; 4.4 DNA-Flavonol Interactions; 4.4.1 Background; 4.4.2 Duplex DNA-Flavonol Interactions.

Reviews in Fluorescence 2016, the tenth volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the yearℓ́ℓs progress in fluorescence and its applications, with authoritative reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. Reviews in Fluorescence offers an essential reference material for any research lab or company working in the fluorescence field and related areas. All academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in the continuously emerging field of fluorescence will find it an invaluable resource.

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