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Preface; Contents; Contributors; Part I: Fundamentals and Analytical Methodologies in Metabolomics; Chapter 1: Metabolomics: Definitions and€Significance in€Systems Biology; 1.1 A Brief History of€Metabolomics and€Its Relevance in€Systems Biology; 1.2 Definitions and€the€Metabolomics Workflow; 1.3 The Importance of€Metabolomics in€Clinical Studies; 1.4 References; Chapter 2: Collection and€Preparation of€Clinical Samples for€Metabolomics; 2.1 Introduction; 2.1.1 Sample Types Investigated in€Clinical Studies and€Associated Considerations; 2.1.2 Sampling Considerations; 2.2 Biofluids.

2.2.1 Urine2.2.1.1 Sample Collection; 2.2.1.2 Storage and€Stability of€Urine Samples; 2.2.1.3 Sample Preparation; 2.2.1.4 Pre-analysis Normalisation; 2.2.2 Serum and€Plasma; 2.2.2.1 Sample Collection; 2.2.2.2 Storage and€Stability; 2.2.2.3 Sample Preparation; 2.2.3 Other Biofluids; 2.2.3.1 Saliva; 2.2.3.2 CSF; 2.2.3.3 Sweat and€Breast Milk; 2.3 Primary and€Immortalised Cells; 2.3.1 Considerations; 2.3.2 Sampling and€Extraction; 2.3.3 Normalisation; 2.4 Tissues; 2.4.1 Sample Collection, Storage and€Stability; 2.4.2 Sample Preparation; 2.4.3 Pre-analysis Normalisation.

2.5 Cell and€Tissue Imaging2.6 Studies Without the€Need for€Sampling; References; Chapter 3: Nuclear Magnetic Resonance Strategies for€Metabolic Analysis; 3.1 Introduction; 3.2 NMR of€Biological Samples; 3.2.1 Biofluids: Plasma/Serum (Blood-Derived Samples); 3.2.2 Biofluids: Urine; 3.2.3 Tissue/Cells: Extraction Procedures; 3.2.4 Tissue: HR-MAS Samples; 3.3 NMR Pulse Sequences; 3.3.1 1D 1H NMR Spectroscopy; 3.3.2 2D J-Resolved NMR Spectroscopy; 3.3.3 2D 1H-1H-TOCSY NMR Spectroscopy; 3.3.4 2D 1H-13C-HSQC NMR Spectroscopy; 3.4 Data Preprocessing; 3.4.1 Automated NMR Spectra Processing.

3.4.2 Baseline Correction3.4.3 Alignment; 3.4.4 Binning; 3.4.5 Data Normalization; 3.5 NMR Databases for€Metabolic Research; 3.5.1 HMDB; 3.5.2 Madison Metabolomics Consortium Database (MMCD); 3.5.3 BioMagResBank; 3.5.4 BML-NMR; 3.5.5 COLMAR; 3.6 Metabolic Analysis of€Kidneys Prior to€Transplantation Using NMR Techniques; 3.6.1 Introduction to€Transplantation; 3.6.2 Metabolism During Organ Preservation; 3.6.3 Biomarker Studies; 3.6.4 Metabolic Pathway Analysis and€2D Studies; 3.6.5 Conclusion; References.

Chapter 4: Metabolomic Strategies Involving Mass Spectrometry Combined with€Liquid and€Gas Chromatography4.1 Introduction; 4.2 Mass Spectrometry; 4.2.1 Mass Analysers Used for€Targeted Metabolomics; 4.2.2 Mass Analysers Used for€Untargeted Metabolomics; 4.3 Liquid Chromatography Coupled to€Mass Spectrometry in€Clinical Metabolomics; 4.3.1 MS Ionisation Techniques Used in€LC-MS; 4.4 Gas Chromatography Coupled to€Mass Spectrometry in€Clinical Metabolomics; 4.5 Conclusions and€Future Prospects; References.

This book provides a comprehensive view of metabolomics, from the basic concepts, through sample preparation and analytical methodologies, to data interpretation and applications in medicine. It is the first volume to cover metabolomics clinical applications while also emphasizing analytical and statistical features. Moreover, future trends and perspectives in clinical metabolomics are also presented. For researches already experienced in metabolomics, the book will be useful as an updated definitive reference. For beginners in the field and graduate students, the book will provide detailed information about concepts and experimental aspects in metabolomics, as well as examples and perspectives of applications of this strategy to clinical questions.