An Anti-Jamming Game When None Player Knows Rival's Channel Gain -- Selfish Mining in Public Blockchains: A Quantitative Analysis -- Improving Sample Efficiency in Evolutionary RL Using Off-Policy Ranking -- Efficiency of Symmetric Nash Equilibria in Epidemic Models with Confinements -- The best of both worlds: Analytically-guided simulation of HPnGs for optimal reachability -- Kubernetes-in-the-Loop: Enriching Microservice Simulation Through Authentic Container Orchestration -- Quantum-enhanced control of a tandem queue system -- Combining Static and Dynamic Traffic with Delay Guarantees in Time-Sensitive Networking -- Caching Contents with Varying Popularity using Restless Bandits -- dSalmon: High-Speed Anomaly Detection for Evolving Multivariate Data Streams -- RealySt: A C++ Tool for Optimizing Reachability Probabilities in Stochastic Hybrid Systems.

This volume contains the proceedings of the 16th EAI International Conference on Performance Evaluation Methodologies and Tools, VALUETOOLS 2023, which took place in Heraklion, Crete during September 6-7, 2023. The conference brought together researchers, developers, and practitioners from around the world and from different communities including computer science, networks and telecommunications, operations research, optimization, control theory, and manufacturing. The 27 members of the International Program Committee (PC) helped to provide at least 3 reviews for each of the 30 submitted contributions. Based on the reviews and PC discussions, 11 high-quality papers (9 research papers, 1 tool paper, and 1 work-in-progress paper) were accepted to be presented during the conference. The volume includes contributions organized into four thematic sessions: Games and Optimization; Simulation; Networking and Queues; Tools.