Brown Adipose Tissue: A Short Historical Perspective -- Brown Fat Anatomy in Humans and Rodents -- Analysis of Thermogenesis Experiments with CalR -- A Clearing Method for Three-Dimensional Imaging of Adipose Tissue -- Activating Human Adipose Tissue with the β3-Adrenergic Agonist Mirabegron -- Fluorescent and Luminescent Methods to Detect Lipolysis -- Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice -- Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes -- Activation of UCP1-Independent Ca2+ Cycling Thermogenesis by Wireless Optogenetics -- Measurement of Futile Creatine Cycling Using Respirometry -- Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes -- Diet-Induced Thermogenesis: Principles and Pitfalls -- Fluorescent Genetic Tools for Studying Brown Fat Development and Function in Mice -- Isolation and Characterization of Human Brown Adipocytes -- Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The 'Goldilocks Solution' -- Signaling Lipidomic Analysis of Thermogenic Adipocytes -- Blood Sampling for Arteriovenous Difference Measurements Across Interscapular Brown Adipose Tissue in Rat -- In Vivo Imaging of Brown Adipose Tissue in Humans: FDG-PET/CT and Beyond -- Analysis of Single Cell/Nucleus Transcriptome Data in Adipose Tissue.

This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and 'omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brown Adipose Tissue: Methods and Protocols provides perspectives and detailed protocols for the benefit of both new BAT researchers looking for guidance as well as seasoned researchers who would like to expand their toolkits. Chapter 12 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.