Deriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos using Physical Distancing and Signal Inhibition -- Induction of Human Naïve Pluripotency Using 5i/L/A Medium -- Induction of Human Naïve Pluripotency Using Chemical Resetting -- Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells -- Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells -- Maintenance of Human Naïve Pluripotent Stem Cells -- Generating Trophoblast Stem Cells from Human Naïve Pluripotent Stem Cells -- Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem Cells -- Capacitation of Human Naïve Pluripotent Stem Cells -- Generation of Pericytic-Vascular Progenitors from Tankyrase/PARP-Inhibitor-Regulated Naïve (TIRN) Human Pluripotent Stem Cells -- Profiling DNA Methylation in Human Naïve Pluripotent Stem Cells -- Chromatin Profiling of Human Naïve Pluripotent Stem Cells -- Mapping Chromatin Accessibility in Human Naïve Pluripotent Stem Cells using ATAC-seq -- Transcriptomic Analysis of Human Naïve and Primed Pluripotent Stem Cells -- Study of X Chromosome Activity Status in Human Naïve Pluripotent Stem Cells using RNA-FISH -- Flow Cytometry Analysis of Cell-Surface Markers to Identify Human Naïve Pluripotent Stem Cells -- Characterizing the Genetic Stability of Human Naïve and Primed Pluripotent Stem Cells.

This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.