CRISPR Library Screening in Cultured Cardiomyocytes -- Protein and mRNA Quantification in Small Samples of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates -- Self-Assembled Heterotypic Cardiac Spheroids from Human Pluripotent Stem Cells -- Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct -- FRESH 3D Bioprinting a Ventricle-Like Cardiac Construct Using Stem Cell-Derived Cardiomyocytes -- Engineered Heart Tissues for Contractile, Structural, and Transcriptional Assessment of Human Pluripotent Stem Cell-Derived Cardiomyocytes in a Three-Dimensional, Auxotonic Environment -- High-Throughput Analysis of Drug Safety Responses in Induced Pluripotent Stem Cell-Derived Cardiomyocytes Using Multielectrode Array -- iPSC-Derived Micro-Heart Muscle for Medium Throughput Pharmacology and Pharmacogenomic Studies -- Quantifying Propagation Velocity from Engineered Cardiac Tissues with High-Speed Fluorescence Microscopy and Automated Analysis Software -- Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte-Fibroblast Microtissues -- Human Engineered Atrial Tissue for Studying Atrial Fibrillation -- Design and Fabrication of Biological Wires for Cardiac Fibrosis Disease Modeling -- Methods for Transepicardial Cell Transplantation in a Swine Myocardial Infarction Model -- Defined Engineered Human Myocardium for Disease Modeling, Drug Screening, and Heart Repair -- Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure -- Quantifying Cardiomyocyte Proliferation and Nucleation to Assess Mammalian Cardiac Regeneration -- Injectable ECM Scaffolds for Cardiac Repair -- Encapsulation of Pediatric Cardiac-Derived C-Kit+ cells in Cardiac Extracellular Matrix Hydrogel for Echocardiography-Directed Intramyocardial Injection in Rodents -- Characterization of the Monocyte Response to Biomaterial Therapy for Cardiac Repair -- Right Ventricular Outflow Tract Surgical Resection in Young, Large Animal Model for the Study of Alternative Cardiovascular Patches.

This detailed volume presents an updated collection of state-of-the-art protocols in cardiac tissue engineering. These protocols demonstrate advancements in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New animal models, biomaterials, and quantitative analyses are described for broad adoption. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols, Second Edition serves as an ideal resource for inspiring the advancement of cardiotoxicity assessment, drug discovery, and heart repair and regeneration in order to accelerate heart health improvement around the globe.