Single-Cell RNA-Sequencing of Somatosensory Neurons -- Preparation of Human and Rodent Spinal Cord Nuclei for Single-Nucleus Transcriptomic Analysis -- Multiplex In Situ Hybridization of the Primate and Rodent DRG and Spinal Cord -- Using Translating Ribosome Affinity Purification (TRAP) to Understand Cell-Specific Translatomes in Pain States -- Ex Vivo Skin-Teased Fiber Recordings from Tibial Nerve -- Single Unit Electrophysiological Recordings of Primary Muscle Sensory Neurons using a Novel Ex Vivo Preparation -- Electrophysiological Recording Techniques from Human Dorsal Root Ganglion -- Human Pluripotent Stem Cell-Derived Sensory Neurons: A New Translational Approach to Study Mechanisms of Sensitization? -- Intraspinal Transplantation of Precursors of Cortical GABAergic Interneurons to Treat Neuropathic Pain -- A Co-Culture System for Studying Dorsal Spinal Cord Synaptogenesis -- Visualizing Synaptic Connectivity using Confocal and Electron Microscopy: Neuroanatomical Approaches to Define Spinal Circuits -- Using Viral Vectors to Visualize Pain-Related Neural Circuits in Mice -- Recording Pain-Related Brain Activity in Behaving Animals using Calcium Imaging and Miniature Microscopes -- Optical Imaging of the Spinal Cord for the Study of Pain: From Molecules to Neural Networks -- In Vivo Calcium Imaging of Peripheral Ganglia -- Optogenetic Modulation of the Visceromotor Response to Reveal Visceral Pain Mechanisms -- Use of Optogenetics for the Study of Skin-Nerve Communication -- Channelrhodopsin-2 Assisted Circuit Mapping in the Spinal Cord Dorsal Horn -- Production of AAVs and Injection into the Spinal Cord -- The Use of Intraspinally Injected Chemogenetic Receptor, PSAM-GlyR, to Probe the Functional Role of Spinal Dorsal Horn Neurons -- Measuring Mouse Somatosensory Reflexive Behaviors with High-Speed Videography, Statistical Modeling, and Machine Learning.

This volume contains experimental approaches that are currently revolutionizing our understanding of the neurobiology of pain. The chapters cover many cutting-edge methods including the identification of gene expression profiles, transcriptomes or translatomes, from individual cells or defined groups of cells in rodents and primates; the electrophysiological investigation of human tissues, such as human dorsal root ganglion neurons; ways to assess modality response profiles of neurons using calcium imaging in vitro and in vivo; and somatosensory behaviors in rodents using high-speed videography and machine learning. In the Neuromethods series style, the chapters include detailed advice from specialists to obtain successful results in your laboratory. Cutting-edge and comprehensive, Contemporary Approaches to the Study of Pain: From Molecules to Neural Networks is a valuable resource for scientists and researchers interested in making impactful contributions to our understanding of pain.