Photo and Soft Lithography for Organs-on-Chip applications -- Organs-on-chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices -- Thermoplastic microfluidics -- Bile Duct-On-a-Chip -- Establishment of a Modular Anaerobic Human Intestine Chip -- Intestinal Epithelium Tubules on a Chip -- A Human Neurovascular Unit On-a-chip -- Generation of a Glomerular Filtration Barrier on a Glomerulus-on-a-chip Platform -- Electro-Mechanical Stimulation of 3D Cardiac Microtissues in a heart-on-chip Model -- Miniaturized Vascularized Bleeding Model of Hemostasis -- Fabrication and Use of a Pumpless Microfluidic Lymphatic Vessel Chip -- On Chip-Platelet Activation Assessment: Microfluidic Emulation of Shear Stress Profiles Induced by Mechanical Circulatory Support Devices -- Biofabrication of 3D Human Muscle Model with Vascularization and Endomysium -- Mechanical induction of Osteoarthritis traits in a cartilage-on-chip model -- A Synovium/Cartilage-On-A-Chip Model To Investigate The Process Of Monocyte Extravasation -- A Mesoscale 3D Culture System For Native And Engineered Biphasic Tissues: Application To The Osteochondral Unit -- 3D biofabricated in Vitro Models of Vascularized and Mineralized Bone Tissues -- Isolation, Integration, and Culture of Human Mature Adipocytes Leveraging Organ-On-Chip Technology. .

This book provides a collection of microphysiological systems employed for chemical/drug screening and strategies to mimic various physiological conditions. Chapters guide readers through Organ-on-a-Chip( OoC) platforms such as liver, intestine, blood-brain barrier, kidney, vessels, cardiac and skeletal muscles, articular joint, human fat. Additional chapters detail microfabrication technologies used to fabricate OoC devices such as, standard photo- and soft-lithography, techniques to fabricate membranes, and industrial-oriented fabrication methods.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Organ-On-a Chip: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.