Purification of Chemokines from Natural Sources -- Cloning of Novel Chemokines Using a Signal Sequence Trap Method -- Chemokine Expression in Insect Cells -- Expression of Chemokines in Escherichia coli -- Expression of Chemokines in the Periplasmic Space of E. coli -- Synthesis of Chemokines -- Identification of Novel Chemokines From Expressed Sequence Tag Databases -- Purification of Recombinant Chemokines from E. coli -- Chemokine Receptor Cloning -- Generation of Stable Cell Lines Expressing Chemokine Receptors -- Modified Microchemotaxis Assays -- Transwell Chemotaxis -- Endothelial Cell Chemotaxis Assays -- Radiolabeled Chemokine Binding Assays -- Scintillation Proximity Binding Assay -- Calcium Mobilization -- Actin Polymerization -- Reactive Oxygen Release -- Histamine Release -- Measurement of Phosphoinositide 3-Kinase Activity -- Glycosaminoglycan Binding Assays -- CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoietic Progenitors -- Downmodulation and Recycling of Chemokine Receptors -- Analysis of Chemokine Receptor Endocytosis and Recycling -- Chemokine Inhibition of HIV Infection -- 26The Production of Chemokine Specific Monoclonal Antibodies -- Monoclonal Antibodies to Chemokine Receptors -- Targeted Expression of Chemokines In vivo -- Chemokine Knockout Mice -- Chemokine Receptor Knockout Mice -- Measurement of Eosinophil Accumulation In vivo -- Murine Model of Allergic Lung Inflammation -- Murine Models of Airway Inflammation -- Rat Models of Respiratory Inflammation -- Murine Model of Crescentic Nephritis -- Rabbit Models of Pneumonia, Peritoneal Sepsis, and Lung Injury.

In the past decade research has established the biological importance of chemokines: they play a major role in leukocyte trafficking, in the recruitment of leukocytes to inflammatory sites, and are coreceptors along with CD4 for HIV cell entry. In Chemokine Protocols, expert investigators describe in detail important techniques usedchemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins.