G-Quadruplexes: From Guanine Gels to Chemotherapeutics -- Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes -- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential -- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis -- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay -- Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy -- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures -- 2-Aminopurine as a Probe for Quadruplex Loop Structures -- Assessing DNA Structures with 125I Radioprobing -- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques -- Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments -- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC -- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography -- Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes -- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands.

Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In G-Quadruplex DNA: Methods and Protocols, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, G-Quadruplex DNA: Methods and Protocols promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.