General -- Fluorescent Measurement of [Ca2+]c -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1 -- Specialist Measurement Systems -- Confocal Microscopy -- Measurement of Intracellular Calcium Concentration Using Confocal Microscopy -- Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i) -- Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR) -- Nonelectrophysiological measurement of Ca2+ channel activity -- Measurement of Ca2+ Entry Using 45Ca2+ -- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding -- Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores -- Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells -- Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay -- Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells -- Photolysis of Caged Calcium Using a Low-Cost Flash Unit -- Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology) -- Continuous Fluorescent Monitoring of Cellular Calcium Fluxe -- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators -- Specialist Measurement Techniques -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Measurement of Calcium and Movement in Heart Cells -- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Ca2+ Sensitive Targets -- Assay and Purification of Calmodulin-Dependent Protein Kinase -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).

In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods-largely based on fluorescence measurement-to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. In addition, there are methods to assess Ca2+ -sensitive target site activity. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin. These cutting-edge techniques will be of enormous value to all those working to understand not only cell signaling, but also the mode of action of a range of pharmacological agents.