Analysis of Nicotinic Acetylcholine Receptor (nAChR) Gene Expression in the Zebrafish (Danio rerio)by In Situ Hybridization and PCR -- Zebrafish: An Animal Model to Study Nicotinic Drugs on Spatial Memory and Visual Attention -- Conditioned Place Preference and Behavioral Analysis to Evaluate Nicotine Reinforcement Properties in Zebrafish -- Study of the Contribution of Nicotinic Receptors to the Release of Endogenous Biogenic Amines in Drosophila Brain -- Emerging Technologies in the Analysis of C. Elegans Nicotinic Acetylcholine Receptors -- Using Natural Genetic Variability in Nicotinic Receptor Genes to Understand the Function of Nicotinic Receptors -- Nicotinic Acetylcholine Receptors as Targets for Tobacco Cessation Therapeutics: Cutting-edge Methodologies to Understand Receptor Assembly and Trafficking -- Spectral Confocal Imaging to Examine Upregulation of Nicotinic Receptor Subunits in α4-Yellow Fluorescent Protein Knock-in Mice -- α7-nicotinic Acetylcholine Receptors: New Therapeutic Avenues in Alzheimer's Disease -- α7-nicotinic Acetylcholine Receptors and β-amyloid Peptides in Alzheimer's Disease -- Development of Antidepressant Drugs Through Targeting a4β2-Nicotinic Acetylcholine Receptors -- Evolutionary Relationship of Nicotinic Acetylcholine Receptor Subunits in Both Vertebrate and Invertebrate Species. .

This volume provides properties, biological function, methods, and approaches for manipulating nAChRs in different organisms. Nicotinic Acetylcholine Receptor Technologies guides readers through molecular techniques and behavioral tests used to investigate nicotinic drugs, chronoamperometry, emerging technologies and methods for the analysis of nAChRs, single nucleotide polymorphism (SNP), fluorescence techniques, spectral confocal, allosteric modulators of a7-nAChRs, and a comprehensive evolutional relation for most nAChR subunits, in both vertebrate and invertebrate species. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical, Nicotinic Acetylcholine Receptor Technologies aims to ensure successful results in the further study of this vital field.