Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids -- Isolation of Photosystem I Particles From Spinach -- Rapid Isolation and Purification of Photosystem I Chlorophyll-Binding Protein From Chlamydomonas reinhardtii -- Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants -- Isolation of Functional Photosystem II Core Particles From the Cyanobacterium Synechocystis sp. PCC 6803 -- Isolation of Photosystem I Reaction Center Preparation From Spinach -- Isolation of Photosystem II Reaction Center Complexes From Plants -- Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach -- Purification and Crystallization of the Cytochrome b 6 f Complex in Oxygenic Photosynthesis -- Purification of Plastocyanin and Cytochrome c 6 From Plants, Green Algae, and Cyanobacteria -- Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex -- Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies With Light-Inducible Structural Transitions -- Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II -- Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants -- The Determination and Quantification of Photosynthetic Pigments by Reverse Phase High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Spectrophotometry -- Isolation and Identification of Chloroplast Lipids -- DNA Adducts With Chlorophyll and Chlorophyllin As Antimutagenic Agents -- Incorporation and Analysis of LHCII in Model Systems -- Photosystem II Reconstitution Into Proteoliposomes -- Extraction of the Functional Manganese and Calcium From Photosystem II -- Assay of Photoinhibition of Photosystem II and Protease Activity -- Thermoluminescence -- Detection of Free Radicals and Reactive Oxygen Species -- Stabilization of Photosynthetic Materials -- Determination of Phosphoproteins in Higher Plant Thylakoids -- Identifying Photoprotection Mutants in Arabidopsis thaliana -- A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii -- The Construction of Gene Knockouts in the Cyanobacterium Synechocystis sp. PCC 6803 -- Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation.

Because photosynthesis is at the heart of plant bioenergetics, fundamental to plant productivity and biomass, and a major factor in global climate, it is widely studied across the many fields it embraces. In Photosynthesis Research Protocols, well-known researchers describe in step-by-step detail a broad range of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. The methods presented are fundamental for research work in agriculture, biochemistry, biotechnology, botany, cell biology, environmental science, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, and plant physiology. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Photosynthesis Research Protocols offers both newcomers and experienced researchers an up-to-date collection of readily reproducible techniques essential for photosynthesis research today.