Genome and Transcript Analyses -- Epstein-Barr Virus -- Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR -- Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids -- Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs) -- Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells -- Visualizing EBV Expression Patterns by FISH -- Viral Detection -- In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products -- Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections -- Detection of EBV Infection at the Single-Cell Level -- Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo -- Culture Methods -- Virus Isolation -- Generation of Lymphoblastoid Cell Lines (LCLs) -- Cell Cycle Distribution of B-Lymphocytes and Cell Lines -- of Plasmid Vectors into Cells Via Electroporation -- Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene -- Transient Gene Expression and MACS Enrichment -- In Vitro Assays to Study Epithelial Cell Growth -- In Vitro Assays to Study Epithelial Cell Differentiation -- Cell Sensitivity Assays -- Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation -- Immune Assays -- Regression Assay -- Generation of Polyclonal EBV-Specific CTL Cultures and Clones -- Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs -- Limiting Dilution Assay -- Viral Protein Detection -- Antibodies for Detecting EBV Latent Proteins -- Detection of EBV Latent Proteins by Western Blotting -- Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis -- Protein-Protein, Protein-DNA, and Protein-RNA Interactions -- The Yeast Two-Hybrid Assay to Identify Interacting Proteins -- Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins -- Magnetic DNA Affinity Purification of a Cellular Transcription Factor -- Screening an Expression Library with a DNA Binding Site -- Analysis of DNA Binding Proteins by Mobility Shift Assay -- Analysis of RNA-Protein Interactions of the EBV-Encoded Small RNAs, the EBERs -- Protein Activity Assays -- Chimeric and Mutated Variants of LMP1 -- Assaying the Activity of Kinases Regulated by LMP1 -- In Vitro Assays for the Detection of Protein Tyrosine Phosphorylation and Protein Tyrosine Kinase Activities -- Tissue and In Vivo Protocols -- Analysis of Apoptosis in Tissue Sections -- Considerations in Generating Transgenic Mice -- In Vivo Assay of Cellular Proliferation -- Topical Chemical Carcinogen Treatment in Mice -- Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture -- Selection and Enrichment of B Cells from Lymphoid Tissues -- Detection of Immunoglobulin Gene Rearrangements.

The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer.