Before In Vivo Imaging: Evaluation of Fluorescent Probes using Fluorescence Microscopy, Multiplate Reader, and Cytotoxicity Assays -- Bioconjugation Methods for Coupling Targeting Ligands with Fluorescent Dyes -- In Vivo Fluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models -- FRET Imaging of Enzymatic Activities Using Smart Probes -- Fluorescence Imaging of Inflammation in Live Animals -- Fluorescence Lifetime Imaging of Cancer In Vivo -- Non-Invasive Imaging of Fluorescent Reporters in Small Rodent Models Using Fluorescence Molecular Tomography -- In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes -- Intraoperative Fluorescence Imaging and Multimodal Surgical Navigation Using Goggle System -- Utilization of Near-Infrared Fluorescent Imaging for Pharmaceutically Relevant Applications -- In Vivo Cell Tracking Using Two-Photon Microscopy -- Small Animal Imaging Using Diffuse Fluorescence Tomography -- In Vivo Metal Ion Imaging Using Fluorescent Sensors -- Using Fluorescence Imaging to Track Drug Delivery and Guide Treatment Planning In Vivo -- In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes -- Ex Vivo Imaging, Biodistribution, and Histological Study in Addition to In Vivo Imaging -- Experimental Design and Data Analysis of In Vivo Fluorescence Imaging Studies.

This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapters on the emerging fields of cell tracking, image-guided treatment, and fluorescence imaging in the second NIR window, as well as protocols for evaluation methods before and after in vivo imaging. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Vivo Fluorescence Imaging: Methods and Protocols serves as a valuable reference for researchers from numerous fields who wish to become more familiar with in vivo fluorescence imaging techniques.