Historical Background on Gamete and Embryo Cryopreservation -- Utility of Animal Models for Human Ovarian Tissue Cryopreservation -- Current Challenges in Immature Oocyte Cryopreservation -- Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification -- Slow Freezing of Human Sperm -- Technology of Aseptic Cryoprotectant-free Vitrification of Human ICSI Spermatozoa -- Human Epididymal and Testicular Sperm Cryopreservation -- Human Oocytes Slow-rate Freezing: Methodology -- Slow Freezing and Thawing of Human Cleavage Stage Embryos -- Human Oocyte Vitrification -- Human Embryo Vitrification -- Human Ovarian Tissue Slow Freezing -- Human Ovarian Tissue Vitrification -- Establishing an Oocyte Cryo Bank Network -- Development of a Nationwide Network for Ovarian Tissue Crypreservation -- Directional Freezing of Ovarian Tissue and Freeze-drying of Stem Cells for Fertility Preservation -- Sterile Plate-Based Vitrification of Adherent Human Pluripotent Stem Cells and Their Derivatives Using the TWIST Method -- Vitrification: A Reliable Method for Cryopreservation of Animal Embryos -- Cryopreservation Effect on Genetic Function: Neonatal Outcomes -- Gavi-Automated Vitrification Instrument -- A. Cryotec® Vitrification Thawing -- B. Solid Surface Vitrification -- C. Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Device -- D. Irvine Scientific® Vitrification System -- E. Rapid-i: Closed Vitrification Device by Vitrolife -- F. Quinn's Advantage Embryo Freeze Kit -- G. Vitrification of Blastocysts Using VitriBlast™ and ThermoBlast™ - Nidacon.

This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.