Enabling Technologies -- Setting Up a New Cell Culture Laboratory -- Adaptation of Mammalian Cells to Growth in Serum-Free Media -- Viral Evaluation of Animal Cell Lines Used in Biotechnology -- Molecular Methods -- Optimizing Gene Expression in Mammalian Cells -- Cytogenetic Characterization of Recombinant Cells -- Immortalization Strategies for Mammalian Cells -- Mouse Monoclonal Antibodies -- Cell Bank Preparation and Characterization -- DNA Fingerprinting and Characterization of Animal Cells -- Cell Evaluation Protocols -- Cell Counting and Viability Measurements -- Monitoring Animal Cell Growth and Productivity by Flow Cytometry -- Measurement of Cell Death in Culture -- Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolism -- Specialist Techniques -- Culturing Animal Cells in Fluidized Bed Reactors -- GPI-Anchored Fusion Proteins -- Harvesting GPI-Anchored Proteins From CHO Cells -- Hematopoietic Cells for Cellular and Gene Therapy -- Hematopoietic Cells for Cellular and Gene Therapy -- Cytotoxicity Testing Using Cell Lines -- Product Evaluation Protocols -- Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells -- Control of Proteolysis in Cell Culture -- Monitoring Recombinant Glycoprotein Heterogeneity -- Measurement of Cell-Culture Glycosidase Activity.

In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases.