Neuronal Death and Neuroprotection: A Review -- The Use of Propidium Iodide to Assess Excitotoxic Neuronal Death in Primary Mixed Cortical Cultures -- A Modified Silver Technique (de Olmos Stain) for Assessment of Neuronal and Axonal Degeneration -- Model of Acute Injury to Study Neuroprotection -- Organotypic Entorhino-Hippocampal Slice Cultures-A Tool to Study the Molecular and Cellular Regulation of Axonal Regeneration and Collateral Sprouting In Vitro -- Role of Nrf2-Dependent ARE-Driven Antioxidant Pathway in Neuroprotection -- Biochemical Methods to Assess the Coupling of Brain Energy Metabolism in Control and Disease States -- A Method for Isolating Prosurvival Targets of NF-?B/Rel Transcription Factors -- Functional Cloning of Genes Regulating Apoptosis in Neuronal Cells -- Characterization of mRNA Expression in Single Neurons -- Brain on a Chip: A Method to Detect Novel Neuroprotective Candidate Targets -- Intracerebral Infusion of Neurotrophic Factors -- Synthesis of Cell-Penetrating Peptides and Their Application in Neurobiology -- The Stem Cells as a Potential Treatment for Neurodegeneration -- In Vivo Tomographic Imaging Studies of Neurodegeneration and Neuroprotection: A Review.

Due to an aging population, the incidence of age-related neurodegenerative diseases is expected to rise dramatically. In Neuroprotection Methods and Protocols, a team of skilled, renowned researchers provide step-by-step laboratory protocols intended to slow or prevent the progression of such diseases. Focusing on neuroprotective approaches, this volume describes basic scientific discoveries and the application of such research within different laboratories in a clear and systematic way in order to orient scientists in this complicated field. The chapters follow the Methods in Molecular Biology™ series format, consisting of readily reproducible methods, lists of the necessary materials, and a Notes section which aids the researcher in solving and avoiding technical problems. Comprehensive and easy to use, Neuroprotection Methods and Protocols is the perfect reference for basic neuroscientists hoping to move forward the ever-important field of neuroprotection and neuronal death research. .