Designed 3D DNA Crystals -- Three-Dimensional DNA Nanostructures Assembled from DNA Star Motifs -- Design of Wireframe DNA Nanostructures-DNA Gridiron -- Complex DNA Brick Assembly -- Computer-Aided Design of RNA Origami Structures -- Assembling RNA Nanoparticles -- DNA Functionalization of Nanoparticles -- Purification Techniques for Three-Dimensional DNA Nanostructures -- DNA Nanostructure as Smart Carriers for Drug Delivery -- DNA G-Quadruplex-Based Assay of Enzyme Activity -- Assembly of Multi-Enzyme Cascade on a DNA Origami Nanostructure -- Lipid Membrane Encapsulation of a 3D DNA NanoOctahedron -- DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures -- Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins -- Direct Nanofabrication Using DNA Nanostructure -- Confined Growth of Metal Nanoparticles Within 3D DNA Origami Molds -- DNA-Directed Self-Assembly of Highly Ordered and Dense Single Walled Carbon Nanotube Arrays -- A Proximity-Based Programmable DNA Nanoscale Assembly Line -- DNA Walkers as Transport Vehicles of Nanoparticles Along a Carbon Nanotube Track.

This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book divided into basic DNA and RNA nanostructure design strategies as well as applications utilizing DNA nanostructures, including but not limited to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, 3D DNA Nanostructure: Methods and Protocols provides the most up-to-date tutorial style overviews and technical style protocols to benefit researchers in a wide variety of areas.