The Basics -- Improvements in the Application of Firefly Luciferase Assays -- Visualization of Bioluminescence -- Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone -- Analytical Biochemistry -- Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue -- Bioluminescent Assay of the Guanylates -- Bioluminescent Assay of the Adenylate Energy Charge -- Molecular Biology Tools -- Photorhabdus luminescens luxCDABE Promoter Probe Vectors -- Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome -- Probing for Promoters with Luciferase-Transposons -- Cryopreservation and Reawakening -- Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists -- Cell-Based Assays -- Genotoxic Sensors -- Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response -- Real-Time Reporter of Protein Synthesis Inhibition -- Luminescence-Based Cell Viability Testing -- Phagocyte Chemiluminescence -- Detection of Oxidants Using lux Fusions to Oxidative Stress Promoters -- Environmental Applications -- Luciferase-Based Measurement of Water Contaminants -- Bioluminescence-Based Metal Detectors -- Luminescence Facilitated Detection of Bioavailable Mercury in Natural Waters -- A Panel of Stress-Responsive Luminous Bacteria for Monitoring Wastewater Toxicity -- Organic Contaminant Detection and Biodegradation Characteristics -- Detection of Firefly Luciferase-Tagged Bacteria in Environmental Samples -- Monitoring of GFP-Tagged Bacterial Cells.

Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.