Poly-L-Lysine-Based Gene Delivery Systems -- Targeted Gene Transfer to Liver Using Protein-DNA Complexes -- Receptor-Directed Molecular Conjugates for Gene Transfer -- Gene Transfer into Muscle by Electroporation In Vivo -- Viral Liposomes -- LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery -- Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery -- Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts -- Regulated Expression of Plasmid-Based Gene Therapies -- Photochemical Transfection Light-Induced, Site-Directed Gene Delivery -- Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun -- Preparation of Pseudotyped Retroviral Vector -- Quantitative Measurement of the Concentration of Active Recombinant Retrovirus -- Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System -- Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells -- Genetically Modified Skin Substitutes -- Bioartificial Muscles in Gene Therapy -- Cytokine Gene-Modified Cell-Based Cancer Vaccines -- HIV-Based Vectors -- Packaging Cell System for Lentivirus Vectors Preparation and Use -- Lentiviral Vectors Preparation and Use -- Simian Foamy Virus Vectors -- Recombinant Feline Immunodeficiency Virus Vectors -- Lentivirus Vector Based on Simian Immunodeficiency Virus -- Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus -- Preparation of Helper-Dependent Adenoviral Vectors -- Construction of First-Generation Adenoviral Vectors -- Preparation of Ovine Adenovirus Vectors -- Highly Purified Recombinant Adeno-Associated Virus Vectors -- High-Titer Stocks of Adeno-Associated Virus from Replicating Amplicons and Herpes Vectors -- Herpes Simplex Virus/Adeno-Associated Virus Hybrid Vectors for Gene Transfer to Neurons -- Development of Replication-Defective Herpes Simplex Virus Vectors.

Significant advances in gene transfer technology and the completion of sequencing the human genome offer much renewed hope that gene therapy will provide novel approaches to the treatment of inherited and acquired diseases. In this fully updated and revised 2nd edition of the warmly received Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. The methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. In addition, there are completely revised methods based on retroviruses and adenoviruses, as well as new and promising methods based on lentiviruses and adeno-associated viruses. The quantitative aspects of gene therapy are strongly emphasized, and the book reflects the many significant recent improvements in gene transfer efficiency, gene regulation, and vector production. Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven step-by-step laboratory procedures that enable successful therapeutic application.